Synergism of Bacillus subtilis and Azospirillum brasilense for enhanced N-use efficiency and maize growth: Evidence from 15N isotopic and physiological responses

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorand Climate-
Autor(es): dc.creatorGalindo, Fernando Shintate-
Autor(es): dc.creatorThiengo, Cassio Carlette-
Autor(es): dc.creatorPagliari, Paulo Humberto-
Autor(es): dc.creatorBernardes, João Victor Silva-
Autor(es): dc.creatordos Santos, Gustavo Duprat-
Autor(es): dc.creatorLongato, Pedro Augusto Fedato-
Autor(es): dc.creatorVilela, Lucila de Sousa-
Autor(es): dc.creatorTeixeira Filho, Marcelo Carvalho Minhoto-
Autor(es): dc.creatorLavres, José-
Data de aceite: dc.date.accessioned2025-08-21T15:53:36Z-
Data de disponibilização: dc.date.available2025-08-21T15:53:36Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/ppl.70205-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302061-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302061-
Descrição: dc.descriptionWe explored the impact of inoculating maize (Zea mays L.) seeds with plant growth-promoting rhizobacteria (PGPR - Bacillus subtilis and Azospirillum brasilense), either individually or in combination, under different nitrogen input levels – control, low, average and high N levels (0, 30, 60, 90 and 120 mg L-1 equivalent to between 0 and 240 kg N ha-1) in a greenhouse setting. Leaf- and plant-level biometrics, nutritional, biochemical, and physiological evaluations were supplemented by isotopic methods (15N isotope dilution and natural abundance - δ15N‰) along with root scanning to investigate N acquisition and distribution. Dual inoculation not only enhanced the recovery of applied N but also bolstered nitrogenase activity, leading to increased biological N fixation (BNF) even at an average level of N (120 kg N ha-1). In instances where dual inoculated plants were grown at low (60 kg N ha-1) and average N levels, a cascade effect was observed, such as encompassing root growth stimulation, enhanced fertilizer and soil exploitation, and increased biomass production. Increasing N application to 240 kg N ha-1 reduced plant biomass by 19–47% compared to 120 kg N ha-1, depending on inoculation strategy. This led to downregulation of nitrogenase activity, diminished PGPR efficiency, and a significant decline in BNF. This study shows how a change in the rhizosphere microbial population can influence a myriad of responses inside the plants. Our research provides valuable insights for studies using 15N isotopic and dual inoculation techniques, offering strong potential for practical implementation.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University College of Agricultural and Technological Sciences Department of Crop Production-
Descrição: dc.descriptionUniversity of São Paulo Center for Nuclear Energy in Agriculture-
Descrição: dc.descriptionUniversity of Minnesota Southwest Research and Outreach Center Department of Soil Water and Climate-
Descrição: dc.descriptionSão Paulo State University Department of Plant Health Rural Engineering and Soils-
Descrição: dc.descriptionSão Paulo State University College of Agricultural and Technological Sciences Department of Crop Production-
Descrição: dc.descriptionSão Paulo State University Department of Plant Health Rural Engineering and Soils-
Descrição: dc.descriptionFAPESP: 2020/06354-2-
Idioma: dc.languageen-
Relação: dc.relationPhysiologia Plantarum-
???dc.source???: dc.sourceScopus-
Título: dc.titleSynergism of Bacillus subtilis and Azospirillum brasilense for enhanced N-use efficiency and maize growth: Evidence from 15N isotopic and physiological responses-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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