Calcium hypochlorite cytotoxicity mechanism in fibroblasts and effect on osteoblast mineralization

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorCoaguila-Llerena, Hernán-
Autor(es): dc.creatorOchoa-Rodríguez, Victor Manuel-
Autor(es): dc.creatorPassos Barbieri, Isadora-
Autor(es): dc.creatorRamos, Simone Gusmão-
Autor(es): dc.creatorFaria, Gisele-
Data de aceite: dc.date.accessioned2025-08-21T23:17:20Z-
Data de disponibilização: dc.date.available2025-08-21T23:17:20Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/iej.13983-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301841-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301841-
Descrição: dc.descriptionAim: To determine the cytotoxicity mechanism of 2.5% calcium hypochlorite [Ca(OCl)2] in L929 fibroblasts and the effect of this solution on human osteoblast-like cells (Saos-2) mineralization, compared to that of 2.5% sodium hypochlorite (NaOCl). Methodology: L929 fibroblasts were exposed to Ca(OCl)2 and NaOCl at different dilutions for 10 min. Cell metabolism was assessed by methyl-thiazole-tetrazolium (MTT); lysosome integrity, by neutral red (NR) assay; type of cell death, by flow cytometry (apoptosis/necrosis); cytoskeleton, by actin and α-tubulin fluorescence and cell ultrastructure, by transmission electron microscopy (TEM). The alkaline phosphatase (ALP) activity and mineralized nodule formation were determined in Saos-2 by thymolphthalein release and alizarin red staining (ARS), respectively. The data were analysed by two-way anova and Bonferroni's post-test (α =.05). Results: Ca(OCl)2 promoted higher cell viability and a lower percentage of apoptosis and necrosis than NaOCl (p <.05). Ca(OCl)2 and NaOCl decreased cell metabolism and lysosome integrity, induced the breakdown of microtubules and actin filaments, promoted alterations of rough endoplasmic reticulum and disruption of mitochondrial cristae. Additionally, Ca(OCl)2 did not induce ALP activity and had no effect on mineralized nodules formation. Conclusions: Although Ca(OCl)2 and NaOCl promoted the same cytotoxicity mechanism, Ca(OCl)2 was less cytotoxic than NaOCl. As for ALP activity, no differences were observed between NaOCl and Ca(OCl)2. The production of mineralized nodules induced by Ca(OCl)2 was lower than those induced by NaOCl, but was not different from those induced by the control group.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Pathology Ribeirão Preto Medical School University of São Paulo – USP-
Descrição: dc.descriptionDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University – UNESP-
Descrição: dc.descriptionFAPESP: #2018/24662-6-
Formato: dc.format64-77-
Idioma: dc.languageen-
Relação: dc.relationInternational Endodontic Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcalcium hypochlorite-
Palavras-chave: dc.subjectcell culture techniques-
Palavras-chave: dc.subjectmaterials testing-
Palavras-chave: dc.subjectroot canal treatment-
Palavras-chave: dc.subjectsodium hypochlorite-
Título: dc.titleCalcium hypochlorite cytotoxicity mechanism in fibroblasts and effect on osteoblast mineralization-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.