Standardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenom

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.contributorButantan Institute (BI)-
Autor(es): dc.creatorOliveira Orsi, Ricardo-
Autor(es): dc.creatorZaluski, Rodrigo-
Autor(es): dc.creatorde Barros, Luciana Curtolo-
Autor(es): dc.creatorBarraviera, Benedito-
Autor(es): dc.creatorPimenta, Daniel Carvalho-
Autor(es): dc.creatorFerreira Junior, Rui Seabra-
Data de aceite: dc.date.accessioned2025-08-21T22:25:10Z-
Data de disponibilização: dc.date.available2025-08-21T22:25:10Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/10937404.2023.2300786-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301827-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301827-
Descrição: dc.descriptionAfricanized bees have spread across the Americas since 1956 and consequently resulted in human and animal deaths attributed to massive attacks related to exposure from Argentina to the USA. In Brazil, more than 100,000 accidents were registered in the last 5 years with a total of 303 deaths. To treat such massive attacks, Brazilian researchers developed the first specific antivenom against Africanized honey bee sting exposure. This unique product, the first of its kind in the world, has been safely tested in 20 patients during a Phase 2 clinical trial. To develop the antivenom, a standardized process was undertaken to extract primary venom antigens from the Africanized bees for immunization of serum-producing horses. This process involved extracting, purifying, fractionating, characterizing, and identifying the venom (apitoxin) employing mass spectrometry to generate standardized antigen for hyperimmunization of horses using the major toxins (melittin and its isoforms and phospholipase A2). The current guide describes standardization of the entire production chain of venom antigens in compliance with good manufacturing practices (GMP) required by regulatory agencies. Emphasis is placed upon the welfare of bees and horses during this process, as well as the development of a new biopharmaceutical to ultimately save lives.-
Descrição: dc.descriptionCollege of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Animal Science College of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP)-
Descrição: dc.descriptionFaculty of Veterinary Medicine and Animal Science Federal University of Mato Grosso do Sul (UFMS)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Clinical Research Botucatu Medical School (FMB) CEVAP São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Biochemistry and Biophysics Butantan Institute (BI)-
Descrição: dc.descriptionCollege of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Animal Science College of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Clinical Research Botucatu Medical School (FMB) CEVAP São Paulo State University (UNESP)-
Formato: dc.format73-90-
Idioma: dc.languageen-
Relação: dc.relationJournal of Toxicology and Environmental Health - Part B: Critical Reviews-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAfricanized honeybee-
Palavras-chave: dc.subjectApis mellifera-
Palavras-chave: dc.subjectgood manufacturing practices-
Palavras-chave: dc.subjecthyperimmunization-
Palavras-chave: dc.subjectstandard operating procedures-
Título: dc.titleStandardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenom-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.