Bottom-Up Proteomics Workflow for Studying Multi-organism Systems

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorNorth Carolina State University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBai, Hongxia-
Autor(es): dc.creatorCollins, Leonard B.-
Autor(es): dc.creatorAndré, Marcos Rogério-
Autor(es): dc.creatorBreitschwerdt, Edward B.-
Autor(es): dc.creatorIslam Williams, Taufika-
Data de aceite: dc.date.accessioned2025-08-21T17:24:25Z-
Data de disponibilização: dc.date.available2025-08-21T17:24:25Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/978-1-0716-4298-6_9-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301799-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301799-
Descrição: dc.descriptionIn recent years, discovery proteomics has emerged as a pivotal tool in biological research, especially when studying the intricate relationships among multiple organisms. To delve deeper into these interactions, we pioneered a bottom-up proteomics workflow. Using nanoLC-MS/MS and a label-free quantification method, this work specifically examines the differential protein expression in fleas (Ctenocephalides felis felis) that have been experimentally infected with Bartonella henselae, the causative agent of cat scratch disease (CSD). Our detailed methodology, from protein cleanup to data analysis using the Proteome Discoverer software, is meticulously outlined to aid other researchers in adopting and adapting this workflow for their own multi-organism studies. This versatile protocol serves as a foundational guide for examining multiple proteomes from varied taxonomic lineages, exemplified in our cat-flea-bacterium investigation.-
Descrição: dc.descriptionDepartment of Chemistry North Carolina State University-
Descrição: dc.descriptionMolecular Education Technology and Research Innovation Center (METRIC) North Carolina State University-
Descrição: dc.descriptionLaboratory of Immunoparasitology Department of Pathology Reproduction and One Health Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP), SP-
Descrição: dc.descriptionIntracellular Pathogens Research Laboratory Department of Clinical Sciences The Comparative Medicine Institute College of Veterinary Medicine North Carolina State University-
Descrição: dc.descriptionLaboratory of Immunoparasitology Department of Pathology Reproduction and One Health Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP), SP-
Formato: dc.format119-141-
Idioma: dc.languageen-
Relação: dc.relationMethods in Molecular Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBartonella henselae-
Palavras-chave: dc.subjectBottom-up proteomics-
Palavras-chave: dc.subjectCtenocephalides felis felis (flea)-
Palavras-chave: dc.subjectDifferential protein expression-
Palavras-chave: dc.subjectnanoLC-MS/MS-
Título: dc.titleBottom-Up Proteomics Workflow for Studying Multi-organism Systems-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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