Advances in forensic genetics: Exploring the potential of long read sequencing

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade de Brasília (UnB)-
Autor(es): dc.contributorNortheastern University-
Autor(es): dc.creatorFerreira, Marcel Rodrigues-
Autor(es): dc.creatorCarratto, Thássia Mayra Telles-
Autor(es): dc.creatorFrontanilla, Tamara Soledad-
Autor(es): dc.creatorBonadio, Raphael Severino-
Autor(es): dc.creatorJain, Miten-
Autor(es): dc.creatorde Oliveira, Silviene Fabiana-
Autor(es): dc.creatorCastelli, Erick C.-
Autor(es): dc.creatorMendes-Junior, Celso Teixeira-
Data de aceite: dc.date.accessioned2025-08-21T23:29:02Z-
Data de disponibilização: dc.date.available2025-08-21T23:29:02Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.fsigen.2024.103156-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301660-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301660-
Descrição: dc.descriptionDNA-based technologies have been used in forensic practice since the mid-1980s. While PCR-based STR genotyping using Capillary Electrophoresis remains the gold standard for generating DNA profiles in routine casework worldwide, the research community is continually seeking alternative methods capable of providing additional information to enhance discrimination power or contribute with new investigative leads. Oxford Nanopore Technologies (ONT) and PacBio third-generation sequencing have revolutionized the field, offering real-time capabilities, single-molecule resolution, and long-read sequencing (LRS). ONT, the pioneer of nanopore sequencing, uses biological nanopores to analyze nucleic acids in real-time. Its devices have revolutionized sequencing and may represent an interesting alternative for forensic research and routine casework, given that it offers unparalleled flexibility in a portable size: it enables sequencing approaches that range widely from PCR-amplified short target regions (e.g., CODIS STRs) to PCR-free whole transcriptome or even ultra-long whole genome sequencing. Despite its higher error rate compared to Illumina sequencing, it can significantly improve accuracy in read alignment against a reference genome or de novo genome assembly. This is achieved by generating long contiguous sequences that correctly assemble repetitive sections and regions with structural variation. Moreover, it allows real-time determination of DNA methylation status from native DNA without the need for bisulfite conversion. LRS enables the analysis of thousands of markers at once, providing phasing information and eliminating the need for multiple assays. This maximizes the information retrieved from a single invaluable sample. In this review, we explore the potential use of LRS in different forensic genetics approaches.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Apoio à Pesquisa do Distrito Federal-
Descrição: dc.descriptionMolecular Genetics and Bioinformatics Laboratory Experimental Research Unit - Unipex School of Medicine São Paulo State University - Unesp, São Paulo-
Descrição: dc.descriptionDepartamento de Química Laboratório de Pesquisas Forenses e Genômicas Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, SP-
Descrição: dc.descriptionDepartamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, SP-
Descrição: dc.descriptionDepto Genética e Morfologia Instituto de Ciências Biológicas Universidade de Brasília, DF-
Descrição: dc.descriptionDepartment of Bioengineering Department of Physics Khoury College of Computer Sciences Northeastern University-
Descrição: dc.descriptionPathology Department School of Medicine São Paulo State University - Unesp, São Paulo-
Descrição: dc.descriptionMolecular Genetics and Bioinformatics Laboratory Experimental Research Unit - Unipex School of Medicine São Paulo State University - Unesp, São Paulo-
Descrição: dc.descriptionPathology Department School of Medicine São Paulo State University - Unesp, São Paulo-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFundação de Apoio à Pesquisa do Distrito Federal: 00193-00002596/2022-40-
Descrição: dc.descriptionCAPES: 307031/2022-5-
Descrição: dc.descriptionCAPES: 310016/2022-3-
Descrição: dc.descriptionCNPq: 408084/2023-5-
Idioma: dc.languageen-
Relação: dc.relationForensic Science International: Genetics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioinformatics-
Palavras-chave: dc.subjectForensic Genetics-
Palavras-chave: dc.subjectMassively Parallel Sequencing-
Palavras-chave: dc.subjectNext-generation sequencing-
Palavras-chave: dc.subjectOxford Nanopore-
Palavras-chave: dc.subjectThird-generation sequencing-
Título: dc.titleAdvances in forensic genetics: Exploring the potential of long read sequencing-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

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