Electrochemical detection of poly(3-hydroxybutyrate) production from Burkholderia glumae MA13 using a molecularly imprinted polymer-reduced graphene oxide modified electrode

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorda Conceição, Emanuela-
Autor(es): dc.creatorBuffon, Edervaldo-
Autor(es): dc.creatorBeluomini, Maísa Azevedo-
Autor(es): dc.creatorFalone, Max Fabrício-
Autor(es): dc.creatorde Andrade, Fernanda Batista-
Autor(es): dc.creatorContiero, Jonas-
Autor(es): dc.creatorStradiotto, Nelson Ramos-
Data de aceite: dc.date.accessioned2025-08-21T19:09:32Z-
Data de disponibilização: dc.date.available2025-08-21T19:09:32Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00604-024-06580-1-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301623-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301623-
Descrição: dc.descriptionThe development and application of an electrochemical sensor is reported for detection of poly(3-hydroxybutyrate) (P3HB) – a bioplastic derived from agro-industrial residues. To overcome the challenges of molecular imprinting of macromolecules such as P3HB, this study employed methanolysis reaction to break down the P3HB biopolymer chains into methyl 3-hydroxybutyrate (M3HB) monomers. Thereafter, M3HB were employed as the target molecules in the construction of molecularly imprinted sensors. The electrochemical device was then prepared by electropolymerizing a molecularly imprinted poly (indole-3-acetic acid) thin film on a glassy carbon electrode surface modified with reduced graphene oxide (GCE/rGO-MIP) in the presence of M3HB. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy with field emission gun (SEM-FEG), Raman spectroscopy, attenuated total reflection Fourier-transform infrared (ATR-FTIR) and X-ray Photoelectron Spectroscopy (XPS) were employed to characterize the electrode surface. Under ideal conditions, the MIP sensor exhibited a wide linear working range of 0.1 – 10 nM and a detection limit of 0.3 pM (n = 3). The sensor showed good repeatability, selectivity, and stability over time. For the sensor application, the bioproduction of P3HB was carried out in a bioreactor containing the Burkholderia glumae MA13 strain and sugarcane byproducts as a supplementary carbon source. The analyses were validated through recovery assays, yielding recovery values between 102 and 104%. These results indicate that this MIP sensor can present advantages in the monitoring of P3HB during the bioconversion process. Graphical Abstract: (Figure presented.)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Pesquisa em Bioenergia (IPBEN) Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Biociências Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionDepartamento de Biotecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Química Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Pesquisa em Bioenergia (IPBEN) Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionInstituto de Biociências Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionDepartamento de Biotecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionFAPESP: 2017/22401-8-
Descrição: dc.descriptionFAPESP: 2018/12131-6-
Descrição: dc.descriptionFAPESP: 2019/13818-8-
Idioma: dc.languageen-
Relação: dc.relationMicrochimica Acta-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomass-
Palavras-chave: dc.subjectBioplastics-
Palavras-chave: dc.subjectDifferential pulse voltammetry-
Palavras-chave: dc.subjectGraphene-
Palavras-chave: dc.subjectMethanolysis-
Palavras-chave: dc.subjectModified glassy carbon electrode-
Palavras-chave: dc.subjectMolecularly imprinted sensor-
Título: dc.titleElectrochemical detection of poly(3-hydroxybutyrate) production from Burkholderia glumae MA13 using a molecularly imprinted polymer-reduced graphene oxide modified electrode-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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