Comparison of the precursor, amino acid oxidation, and end-product methods for the evaluation of protein turnover in senior dogs

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPacheco, Letícia G.-
Autor(es): dc.creatorGoloni, Camila-
Autor(es): dc.creatorDi Santo, Ludmilla G.-
Autor(es): dc.creatorScarpim, Lucas B.-
Autor(es): dc.creatorEugênio, Débora A.-
Autor(es): dc.creatorCastro, Ariel de-
Autor(es): dc.creatorCosta, Vladimir E.-
Autor(es): dc.creatorCarciofi, Aulus C.-
Data de aceite: dc.date.accessioned2025-08-21T22:42:51Z-
Data de disponibilização: dc.date.available2025-08-21T22:42:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0305073-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301606-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301606-
Descrição: dc.descriptionStable isotope methods have been used to study protein metabolism in humans; however, there application in dogs has not been frequently explored. The present study compared the methods of precursor (13C-Leucine), end-products (15N-Glycine), and amino acid oxidation (13C-Phenylalanine) to determine the whole-body protein turnover rate in senior dogs. Six dogs (12.7 ± 2.6 years age, 13.6 ± 0.6 kg bodyweight) received a dry food diet for maintenance and were subjected to all the above-mentioned methods in succession. To establish 13C and 15N kinetics, according to different methodologies blood plasma, urine, and expired air were collected using a specifically designed mask. The volume of CO2 was determined using respirometry. The study included four methods viz. 13C-Leucine, 13C-Phenylalanine evaluated with expired air, 13C-Phenylalanine evaluated with urine, and 15N-Glycine, with six dogs (repetitions) per method. Data was subjected to variance analysis and means were compared using the Tukey test (P<0.05). In addition, the agreement between the methods was evaluated using Pearson correlation and Bland-Altman statistics. Protein synthesis (3.39 ± 0.33 g.kg-0,75. d-1), breakdown (3.26 ± 0.18 g.kg-0.75.d-1), and flux estimations were similar among the four methods of study (P>0.05). However, only 13C-Leucine and 13C-Phenylalanine (expired air) presented an elevated Pearson correlation and concordance. This suggested that caution should be applied while comparing the results with the other methodologies.-
Descrição: dc.descriptionSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute Bioscience Stable Isotope Center São Paulo State University (UNESP)-
Descrição: dc.descriptionSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute Bioscience Stable Isotope Center São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titleComparison of the precursor, amino acid oxidation, and end-product methods for the evaluation of protein turnover in senior dogs-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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