Molecular survey of hemoplasmas and Coxiella burnetii in vampire bats from northern Brazil

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEvandro Chagas Institute MS-SVS-
Autor(es): dc.creatorde Mello, Victória Valente Califre-
Autor(es): dc.creatorde Oliveira, Laryssa Borges-
Autor(es): dc.creatorCoelho, Taciana Fernandes Souza Barbosa-
Autor(es): dc.creatorLee, Daniel Antonio Braga-
Autor(es): dc.creatorFranco, Eliz Oliveira-
Autor(es): dc.creatorMachado, Rosangela Zacarias-
Autor(es): dc.creatorAndré, Marcos Rogério-
Data de aceite: dc.date.accessioned2025-08-21T17:53:31Z-
Data de disponibilização: dc.date.available2025-08-21T17:53:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.cimid.2024.102127-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301472-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301472-
Descrição: dc.descriptionIn addition to zoonotic viral pathogens, bats can also harbor bacterial pathogens, including hemoplasmas (hemotropic mycoplasmas) and Coxiella burnetii. The present study aimed to investigate, using molecular techniques, the presence of hemoplasmas and C. burnetii in spleen samples from vampire bats in northern Brazil. For this purpose, between 2017 and 2019, spleen samples were collected from Desmodus rotundus (n = 228) and Diaemus youngii (n = 1) captured in the states of Pará (n = 207), Amazonas (n = 1), Roraima (n = 18) and Amapá (n = 3). DNA samples extracted from the bat spleen and positive in PCR for the endogenous gapdh gene were subjected to conventional PCR assays for the 16S rRNA, 23S rRNA and RNAse P genes from hemoplasmas and to qPCR based on the IS1111 gene element for C. burnetii. All spleen samples from vampire bats were negative in the qPCR for C. burnetii. Hemoplasmas were detected in 10 % (23/229) of spleen samples using a PCR based on the 16S rRNA gene. Of these, 21.73 % (5/23) were positive for the 23S rRNA gene and none for the RNAseP gene. The seven hemoplasma 16S rRNA sequences obtained were closely related to sequences previously identified in vampire bats from Belize, Peru and Brazil. The 23S rRNA sequence obtained revealed genetic proximity to hemoplasmas from non-hematophagous bats from Brazil and Belize. The analysis revealed different circulating genotypes among Brazilian vampire bats, in addition to a trend towards genera-specific hemoplasma genotypes. The present study contributes to the knowledge of the wide diversity of hemoplasmas in vampire bats.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionPostgraduate Program in Agricultural Microbiology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionSection of Arbovirology and Hemorrhagic Fevers Coordinator of the Rabies Diagnosis Laboratory Evandro Chagas Institute MS-SVS, PA-
Descrição: dc.descriptionPostgraduate Program in Agricultural Microbiology School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2022/08543-2-
Descrição: dc.descriptionCNPq: 303701/2021-8-
Idioma: dc.languageen-
Relação: dc.relationComparative Immunology, Microbiology and Infectious Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGenetic diversity-
Palavras-chave: dc.subjectHemoplasmas-
Palavras-chave: dc.subjectQ fever-
Palavras-chave: dc.subjectVampire bats-
Título: dc.titleMolecular survey of hemoplasmas and Coxiella burnetii in vampire bats from northern Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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