Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Florida-
Autor(es): dc.creatorPedrosa, Valber A.-
Autor(es): dc.creatorChen, Kangfu-
Autor(es): dc.creatorGeorge, Thomas J.-
Autor(es): dc.creatorFan, Z. Hugh-
Data de aceite: dc.date.accessioned2025-08-21T21:20:51Z-
Data de disponibilização: dc.date.available2025-08-21T21:20:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/bios13070706-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301419-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301419-
Descrição: dc.descriptionLiquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% ± 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our method and platform enables the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs for biological assays.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionCenter for Information Technology-
Descrição: dc.descriptionCenter for Scientific Review-
Descrição: dc.descriptionNational Institutes of Health-
Descrição: dc.descriptionOffice of Extramural Research, National Institutes of Health-
Descrição: dc.descriptionOffice of Research Infrastructure Programs, National Institutes of Health-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionMcJunkin Family Charitable Foundation-
Descrição: dc.descriptionInstitute of Bioscience of Botucatu Sao Paulo State University—Unesp-
Descrição: dc.descriptionInterdisciplinary Microsystems Group Department of Mechanical and Aerospace Engineering University of Florida, P.O. Box 116250-
Descrição: dc.descriptionDepartment of Medicine University of Florida, P.O. Box 100278-
Descrição: dc.descriptionJ. Crayton Pruitt Family Department of Biomedical Engineering University of Florida, P.O. Box 116131-
Descrição: dc.descriptionInstitute of Bioscience of Botucatu Sao Paulo State University—Unesp-
Descrição: dc.descriptionFAPESP: 2017/25937-6-
Descrição: dc.descriptionFAPESP: 2019/13411-5-
Descrição: dc.descriptionCNPq: 303016/2022-1-
Descrição: dc.descriptionMcJunkin Family Charitable Foundation: R01CA238387-
Idioma: dc.languageen-
Relação: dc.relationBiosensors-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcell capture-
Palavras-chave: dc.subjectcirculating tumor cells-
Palavras-chave: dc.subjectgold nanoparticles-
Palavras-chave: dc.subjectmicrofluidics-
Título: dc.titleGold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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