Unravelling spermatogenesis in spotted wolffish: Insights from the ultrastructure of juvenile male testes to the cryopreservation of broodstock sperm

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Autor(es): dc.contributorNord University-
Autor(es): dc.contributorUniversity of Oslo-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorBiotechnology and Aquaculture. Hellenic Center for Marine Research-
Autor(es): dc.contributorFram Centre-
Autor(es): dc.contributorEast China Normal University-
Autor(es): dc.contributorUniversity of Murcia-
Autor(es): dc.creatorSuperio, Joshua-
Autor(es): dc.creatorResseguier, Julien-
Autor(es): dc.creatorNobrega, Rafael Henrique-
Autor(es): dc.creatorGrebstad, Caroline M.-
Autor(es): dc.creatorFakriadis, Ioannis-
Autor(es): dc.creatorFoss, Atle-
Autor(es): dc.creatorHagen, Ørjan-
Autor(es): dc.creatorZhang, Meiling-
Autor(es): dc.creatordel Pilar García-Hernández, Maria-
Autor(es): dc.creatorGalindo-Villegas, Jorge-
Data de aceite: dc.date.accessioned2025-08-21T16:29:38Z-
Data de disponibilização: dc.date.available2025-08-21T16:29:38Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.aquaculture.2024.741214-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301387-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301387-
Descrição: dc.descriptionThe aim of this study was to deepen our understanding of the reproductive biology of male spotted wolffish (Anarhichas minor) using two different experimental approaches involving juvenile and mature broodstock fish. The first approach consisted of a detailed histological examination of the testes to identify the onset of gonadal maturation and characterise the spermatogenic stages in two- and three-year-old juvenile specimens. Light microscopy analysis revealed clear differences between the age groups. Two-year-old fish displayed well-defined interstitial tissue, Sertoli cells and cysts housing spermatogonia stem cells in which meiosis had not yet begun. In contrast, three-year-old fish exhibited cysts containing spermatocytes, spermatids and abundant spermatozoa, indicating the initiation of the spermatogenic cycle, albeit with asynchronous puberty. Histochemical staining revealed a significant presence of smooth myoid cells in the interstitial tissue of sexually mature fish, while electron microscopy further revealed synaptonemal complexes indicating the onset of meiosis and centriolar structures that gave rise to flagella. The second approach focused on optimising semen freezing and cryopreservation procedures in mature broodstock individuals over the age of 10 years. Seven freezing extenders (KT, TS-2, OP, MT, MH, HBSS, or SR), with seawater (SW) as a control, were assessed along with two cryoprotectants dimethylsulfoxide (DMSO) or methanol to evaluate their impact on pre- and post-thaw semen quality. Results showed that the MT and HBSS extenders were superior in total sperm kinetics at 1:3 dilution, and that DMSO showed optimal results in sperm motility and velocity variants. Moreover, the MT and HBSS groups demonstrated consistent sperm viability after cryopreservation, with values similar to fresh samples. Based on the viability results of the SYBR-green-14/PI assay comparing fresh and cryopreserved sperm using MT and HBSS, the MT extender emerged as the most effective freezing medium for cryopreservation of spotted wolffish broodstock sperm. In conclusion, this study provides a comprehensive understanding of the reproductive dynamics of male spotted wolffish, offering valuable insights for both scientific research and aquaculture management.-
Descrição: dc.descriptionNorges Forskningsråd-
Descrição: dc.descriptionEuropean Commission-
Descrição: dc.descriptionDepartment of Genomics Faculty of Biosciences and Aquaculture Nord University-
Descrição: dc.descriptionSection for Physiology and Cell Biology Departments of Biosciences and Immunology University of Oslo-
Descrição: dc.descriptionReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), 18618-970 Botucatu-
Descrição: dc.descriptionInstitute of Marine Biology Biotechnology and Aquaculture. Hellenic Center for Marine Research-
Descrição: dc.descriptionAkvaplan-Niva Fram Centre-
Descrição: dc.descriptionDepartment of Aquaculture Faculty of Biosciences and Aquaculture Nord University-
Descrição: dc.descriptionLaboratory of Aquaculture Nutrition and Environmental Health (LANEH) School of Life Sciences East China Normal University-
Descrição: dc.descriptionDepartment of Cell Biology and Histology Faculty of Biology University of Murcia, Campus de Espinardo-
Descrição: dc.descriptionReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), 18618-970 Botucatu-
Descrição: dc.descriptionNorges Forskningsråd: 311799-
Descrição: dc.descriptionEuropean Commission: EU H2020-
Idioma: dc.languageen-
Relação: dc.relationAquaculture-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnarhichas minor-
Palavras-chave: dc.subjectCASA-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectEmerging teleost species-
Palavras-chave: dc.subjectExtenders-
Palavras-chave: dc.subjectPhalloidin-
Palavras-chave: dc.subjectSpermatogenesis-
Título: dc.titleUnravelling spermatogenesis in spotted wolffish: Insights from the ultrastructure of juvenile male testes to the cryopreservation of broodstock sperm-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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