Bioprospecting, Synergistic Antifungal and Toxicological Aspects of the Hydroxychalcones and Their Association with Azole Derivates against Candida spp. for Treating Vulvovaginal Candidiasis

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFernandes, Lígia de Souza-
Autor(es): dc.creatorOgasawara, Letícia Sayuri-
Autor(es): dc.creatorMedina-Alarcón, Kaila Petronila-
Autor(es): dc.creatordos Santos, Kelvin Sousa-
Autor(es): dc.creatorde Matos Silva, Samanta-
Autor(es): dc.creatorde Assis, Letícia Ribeiro-
Autor(es): dc.creatorRegasini, Luís Octavio-
Autor(es): dc.creatorde Oliveira, Anselmo Gomes-
Autor(es): dc.creatorMendes Giannini, Maria José Soares-
Autor(es): dc.creatorScarpa, Maria Virginia-
Autor(es): dc.creatorFusco Almeida, Ana Marisa-
Data de aceite: dc.date.accessioned2025-08-21T20:44:43Z-
Data de disponibilização: dc.date.available2025-08-21T20:44:43Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pharmaceutics16070843-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301333-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301333-
Descrição: dc.descriptionVulvovaginal candidiasis (VVC) remains a prevalent fungal disease, characterized by challenges, such as increased fungal resistance, side effects of current treatments, and the rising prevalence of non-albicans Candida spp. naturally more resistant. This study aimed to propose a novel therapeutic approach by investigating the antifungal properties and toxicity of 2-hydroxychalcone (2-HC) and 3′-hydroxychalcone (3′-HC), both alone and in combination with fluconazole (FCZ) and clotrimazole (CTZ). A lipid carrier (LC) was also developed to deliver these molecules. The study evaluated in vitro anti-Candida activity against five Candida species and assessed cytotoxicity in the C33-A cell line. The safety and therapeutic efficacy of in vivo were tested using an alternative animal model, Galleria mellonella. The results showed antifungal activity of 2-HC and 3′-HC, ranging from 7.8 to 31.2 as fungistatic and 15.6 to 125.0 mg/L as fungicide effect, with cell viability above 80% from a concentration of 9.3 mg/L (2-HC). Synergistic and partially synergistic interactions of these chalcones with FCZ and CTZ demonstrated significant improvement in antifungal activity, with MIC values ranging from 0.06 to 62.5 mg/L. Some combinations reduced cytotoxicity, achieving 100% cell viability in many interactions. Additionally, two LCs with suitable properties for intravaginal application were developed. These formulations demonstrated promising therapeutic efficacy and low toxicity in Galleria mellonella assays. These results suggest the potential of this approach in developing new therapies for VVC.-
Descrição: dc.descriptionLaboratory of Clinical Mycology Department of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Road Araraquara Jaú, Km 01, S/N, SP-
Descrição: dc.descriptionDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Road Araraquara Jaú, Km 01, S/N, SP-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), St. Quirino de Andrade, 215, SP-
Descrição: dc.descriptionLaboratory of Clinical Mycology Department of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Road Araraquara Jaú, Km 01, S/N, SP-
Descrição: dc.descriptionDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Road Araraquara Jaú, Km 01, S/N, SP-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), St. Quirino de Andrade, 215, SP-
Idioma: dc.languageen-
Relação: dc.relationPharmaceutics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCandidaspp-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjecthydroxychalcone-
Palavras-chave: dc.subjectlipid carriers-
Palavras-chave: dc.subjecttoxicity-
Palavras-chave: dc.subjectvulvovaginal candidiasis-
Título: dc.titleBioprospecting, Synergistic Antifungal and Toxicological Aspects of the Hydroxychalcones and Their Association with Azole Derivates against Candida spp. for Treating Vulvovaginal Candidiasis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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