Contribution of lipids to the organelle differential profile of in vitro-produced bovine embryos

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Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.contributorPurdue University-
Autor(es): dc.contributorGenus plc-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Pampa-
Autor(es): dc.creatorAnnes, Kelly-
Autor(es): dc.creatorFerreira, Christina Ramires-
Autor(es): dc.creatorValente, Roniele Santana-
Autor(es): dc.creatorMarsico, Thamiris Vieira-
Autor(es): dc.creatorTannura, Juliana Hayashi-
Autor(es): dc.creatorda Silveira, Juliano Coelho-
Autor(es): dc.creatorSilva, Flávio Henrique-
Autor(es): dc.creatorLandim-Alvarenga, Fernanda da Cruz-
Autor(es): dc.creatorMesquista, Fernando Silveira-
Autor(es): dc.creatorSudano, Mateus José-
Data de aceite: dc.date.accessioned2025-08-21T15:41:07Z-
Data de disponibilização: dc.date.available2025-08-21T15:41:07Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-09-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2023.06.005-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301328-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301328-
Descrição: dc.descriptionEach living organism is unique because of the lipid identity of its organelles. The diverse distribution of these molecules also contributes to the role of each organelle in cellular activity. The lipid profiles of whole embryos are well documented in the literature. However, this approach can often lead to the loss of relevant information at the subcellular and consequently, metabolic levels, hindering a deeper understanding of key physiological processes during preimplantation development. Therefore, we aimed to characterize four organelles in vitro-produced bovine embryos: lipid droplets (LD), endoplasmic reticulum (ER), mitochondria (MIT), and nuclear membrane (NUC), and evaluate the contribution of the lipid species to each organelle evaluated. Expanded blastocysts were subjected to cell organelle isolation. Thereafter, lipid extraction from cell organelles and lipid analysis using the Multiple Reaction Monitoring (MRM) profiling method were performed. The LD and ER displayed a greater number of lipids (Phosphatidylcholine - PC, Ceramide - Cer, and Sphingomielin - SM) with high signal-to-noise intensities. This result is due to the high rate of biosynthesis, lipid distribution, and ability to store and recycle lipid species of these organelles. The NUC had a more distinct lipid profile than the other three organelles, with high relative intensities of PC, SM, and triacylglycerols (TG), which is consistent with its high nuclear activity. MIT had an intermediate profile that was close to that of LD and ER, which aligns with its autonomous metabolism for some classes of phospholipids (PL). Our study revealed the lipid composition of each organelle studied, and the roles of these lipids could be associated with the characteristic organellar activity. Our findings highlight the lipid species and classes that are relevant for the homeostasis and function of each associated organelle and provide tentative biomarkers for the determination of in vitro embryonic development and quality.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of São Carlos, Rod. Washington Luis - Km 235, SP-
Descrição: dc.descriptionCenter of Natural and Human Sciences Universidade Federal do ABC, Av. dos Estados, 5001, SP-
Descrição: dc.descriptionBindley Bioscience Center and Center for Analytical Instrumentation Development Department of Chemistry Purdue University-
Descrição: dc.descriptionGenus plc, SP-
Descrição: dc.descriptionDepartment of Veterinary Medicine Faculty of Animal Sciences and Food Engineering University of Sao Paulo, Sao Paulo-
Descrição: dc.descriptionDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University, SP-
Descrição: dc.descriptionSchool of Veterinary Medicine Federal University of Pampa, BR 472 - KM 592, PO Box 118, RS-
Descrição: dc.descriptionDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University, SP-
Descrição: dc.descriptionFAPESP: 2018/21496-8-
Descrição: dc.descriptionFAPESP: 2020/08826-9-
Formato: dc.format109-118-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEmbryo-
Palavras-chave: dc.subjectLipids-
Palavras-chave: dc.subjectMembrane composition-
Palavras-chave: dc.subjectMetabolomics-
Palavras-chave: dc.subjectOrganelles-
Título: dc.titleContribution of lipids to the organelle differential profile of in vitro-produced bovine embryos-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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