Canine Adipose-Derived Mesenchymal Stromal Cells Reduce Cell Viability and Migration of Metastatic Canine Oral Melanoma Cell Lines In Vitro

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorInstitute of Veterinary Oncology (IOVET)-
Autor(es): dc.contributorVet Precision Laboratory-
Autor(es): dc.creatorTeng, Fwu Shing-
Autor(es): dc.creatorde Faria Lainetti, Patricia-
Autor(es): dc.creatorSimão Franzoni, Mayara-
Autor(es): dc.creatorFernando Leis Filho, Antonio-
Autor(es): dc.creatorde Oliveira Massoco Salles Gomes, Cristina-
Autor(es): dc.creatorLaufer-Amorim, Renée-
Autor(es): dc.creatorMartins Amorim, Rogério-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo-
Data de aceite: dc.date.accessioned2025-08-21T17:43:38Z-
Data de disponibilização: dc.date.available2025-08-21T17:43:38Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/vetsci11120636-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301315-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301315-
Descrição: dc.descriptionCanine oral melanoma (COM) is a promising target for immunomodulatory therapies aimed at enhancing the immune system’s antitumor response. Given that adipose-derived mesenchymal stem cells (Ad-MSCs) possess immunomodulatory properties through cytokine release, we hypothesized that co-culturing Ad-MSCs and canine peripheral blood mononuclear cells (PBMCs) could stimulate interleukin (IL) production against melanoma cell lines (MCCLs) and help identify therapeutic targets. This study evaluated IL-2, IL-8, and IL-12 expressions in co-culture with MCCL, Ad-MSCs, and PBMCs and assessed the relationship between gene expression, cell viability, and migration. Using four experimental groups in a Transwell insert system to separate cell types, we found that Ad-MSCs can reduce MCCL migration and viability, though the effect may vary depending on each cell line’s susceptibility. Furthermore, Ad-MSCs modified IL expression profiles in co-cultured cells. Our findings suggest that Ad-MSCs could have therapeutic potential for COM by inhibiting cell migration and reducing viability. However, deeper insights into Ad-MSC interactions with the tumor microenvironment and melanoma-specific factors will be essential to optimize therapeutic efficacy.-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Pathology School of Veterinary Medicine and Animal Science University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Veterinary Oncology (IOVET)-
Descrição: dc.descriptionVet Precision Laboratory-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectdog-
Palavras-chave: dc.subjectinterleukins-
Palavras-chave: dc.subjectmelanocyte-
Palavras-chave: dc.subjectperipheral mononuclear cells-
Palavras-chave: dc.subjectstem cells-
Título: dc.titleCanine Adipose-Derived Mesenchymal Stromal Cells Reduce Cell Viability and Migration of Metastatic Canine Oral Melanoma Cell Lines In Vitro-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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