Successful cryopreservation in biodegradable containers of sperm from aquaculture Mediterranean fishes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversitat Politècnica de València-
Autor(es): dc.contributorFederal University of Rio Grande do Sul-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCSIC)-
Autor(es): dc.creatorFrança, T. S.-
Autor(es): dc.creatorGonzález-López, W. A.-
Autor(es): dc.creatorSanchez, M. P.-
Autor(es): dc.creatorFerrão, L.-
Autor(es): dc.creatorFernández-García, F.-
Autor(es): dc.creatorBorges, L. P.-
Autor(es): dc.creatorBelenguer, A.-
Autor(es): dc.creatorHolhorea, P. G.-
Autor(es): dc.creatorCalduch-Giner, J. C.-
Autor(es): dc.creatorFelip, A.-
Autor(es): dc.creatorGómez, A.-
Autor(es): dc.creatorPérez-Sánchez, J.-
Autor(es): dc.creatorStreit, D. P.-
Autor(es): dc.creatorAsturiano, J. F.-
Data de aceite: dc.date.accessioned2025-08-21T20:11:19Z-
Data de disponibilização: dc.date.available2025-08-21T20:11:19Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2023.12.016-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301207-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301207-
Descrição: dc.descriptionWe aimed to evaluate the efficiency of hard-gelatin and hard-hydroxypropyl methylcellulose (HPMC) capsules as biodegradable alternative containers to plastic straws in European eel (Anguilla anguilla), gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax) sperm cryopreservation. Sperm samples from each European eel (n = 12) were diluted 1:8:1 (sperm: extender P1+5 % egg yolk: methanol). Gilthead seabream (n = 12) samples were individually diluted in a cryoprotectant solution of 5 % Me2SO + NaCl 1 % plus BSA (10 mg mL−1) at a ratio of 1:6 (sperm: cryoprotectant solution). European sea bass (n = 10) sperm from each male was diluted in non-activating medium (NAM) at a ratio of 1:5.7 (sperm: NAM), and 5 % of Me2SO was added. The diluted European eel and sea bass sperm aliquots (0.5 mL) were individually filled in plastic straws (0.5 mL), hard-gelatin, and HPMC capsules (0.68 mL). Gilthead seabream diluted sperm (0.25 mL) were filled in plastic straws (0.25 mL) and identical capsules described. All samples were frozen in liquid nitrogen vapor and stored in a liquid nitrogen tank. Sperm kinetic parameters were evaluated by CASA-Mot software. Sperm membrane integrity was performed using a Live and Dead KIT and an epifluorescence microscope. To quantify DNA damage, the alkaline comet assay was performed and TailDNA (TD-%) and Olive Tail Moment (OTM) were evaluated by CaspLab software. Sperm cryopreservation of the three Mediterranean species in straws, gelatin, or HPMC capsules reduced the kinetic parameters and cell membrane integrity. Generally, the post-thawing samples cryopreserved in straws and capsules did not differ for the kinetic parameters and cell membrane integrity, except for European sea bass sperm, where the samples stored in gelatin capsules showed higher velocities (VCL - 100; VSL - 76; VAP - 90 μm s−1) than the sperm stored in HPMC capsules (VCL - 87; VSL - 59; VAP - 73 μm s−1). The cryopreservation process did not damage the sperm DNA of European eel and European sea bass, regardless of the containers used. On the other hand, gilthead seabream sperm cryopreserved in gelatin (TD - 9.8 %; OTM - 9.7) and HPMC (TD - 11.1 %; OTM - 11.2) capsules showed higher DNA damage than fresh samples (TD - 3.6 %; OTM - 2.7) and the sperm stored in straws (TD - 4.4 %; OTM - 5.2). The hard-gelatin and HPMC biodegradable capsules can be used as an alternative to straws for European eel, gilthead seabream, and European sea bass sperm cryopreservation.-
Descrição: dc.descriptionGeneralitat Valenciana-
Descrição: dc.descriptionGrupo de Acuicultura y Biodiversidad Instituto de Ciencia y Tecnologia Animal Universitat Politècnica de València, Edificio 7G, Camino de Vera s/n-
Descrição: dc.descriptionAquam Research Group Animal Science Research Program Federal University of Rio Grande do Sul, RS-
Descrição: dc.descriptionNeotropical Ichthyology Laboratory LINEO Group Departament of Biology and Animal Science São Paulo State University UNESP– Univ. Estadual Paulista, Avenida Brasil Centro, 56, Ilha Solteira-
Descrição: dc.descriptionInstitute of Aquaculture Torre de la Sal (IATS CSIC), Ribera de Cabanes-
Descrição: dc.descriptionNeotropical Ichthyology Laboratory LINEO Group Departament of Biology and Animal Science São Paulo State University UNESP– Univ. Estadual Paulista, Avenida Brasil Centro, 56, Ilha Solteira-
Descrição: dc.descriptionGeneralitat Valenciana: THINKINAZUL/2021/012-
Descrição: dc.descriptionGeneralitat Valenciana: THINKINAZUL/2021/024-
Descrição: dc.descriptionGeneralitat Valenciana: THINKINAZUL/2021/042-
Formato: dc.format53-61-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCapsule-
Palavras-chave: dc.subjectCryobiology-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectGelatin-
Palavras-chave: dc.subjectHPMC-
Título: dc.titleSuccessful cryopreservation in biodegradable containers of sperm from aquaculture Mediterranean fishes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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