Decreased expression of microRNA-629 in gastric cancer samples potentiated by the virulence marker of H. pylori, cagA gene

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Autor(es): dc.contributorFaculdade de Medicina de Marília-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorCentro Universitário das Faculdades Integradas de Ourinhos-
Autor(es): dc.creatorSoares, Caroline Dos Reis Rodrigues-
Autor(es): dc.creatorDa Silva, Lucas Matheus Vieira-
Autor(es): dc.creatorAlmeida, Bianca Reis-
Autor(es): dc.creatorPereira, Jéssica Nunes-
Autor(es): dc.creatorSantos, Mônica Pezenatto Dos-
Autor(es): dc.creatorBarbosa, Mônica Santiago-
Autor(es): dc.creatorSmith, Marília De Arruda Cardoso-
Autor(es): dc.creatorPayão, Spencer Luiz Marques-
Autor(es): dc.creatorRasmussen, Lucas Trevizani-
Data de aceite: dc.date.accessioned2025-08-21T19:46:32Z-
Data de disponibilização: dc.date.available2025-08-21T19:46:32Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S0004-2803.24612023-139-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/301005-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/301005-
Descrição: dc.descriptionBackground – Helicobacter pylori (H. pylori) is a gram-negative bacterium associated with the etiology of several gastrointestinal tract pathologies, and cagA-positive (cagA+) strains are found in populations with gastric ulcers and precancerous lesions, inducing pro-inflammatory responses. The development of neoplasms is related to microRNA (miRNA) dysregulation, indicating highly expressed miRNA-629. The article aims to correlate the expression level of miRNA-629 with the presence of H. pylori and the pathogenicity marker cagA. Methods – 203 gastric biopsy samples were evaluated from individuals with normal gastric tissue (n=60), gastritis (n=96), and gastric cancer (n=47) of both genders and over 18 years old. The samples were subdivided according to the presence or absence of H. pylori, detected by polymerase chain reaction (PCR). RNA was extracted using a commercial kit and quantified. Complementary DNA (cDNA) was synthesized using commercial kits, and the relative expression was calculated using the 2-ΔΔCt method. Results – Individuals infected with H. pylori are nine times more likely to develop gastric cancer. Cancer patients appeared to have decreased expression of miRNA-629; however, the presence of the bacterium would not influence this reduction. Individuals in the cancer group showed lower miRNA-629 expression when cagA+; however, in the control group, the expression was higher when cagA+. Conclusion – H. pylori is a factor involved in the etiology and progression of gastric diseases. Reduction in miRNA-629 expression in cancer patients occurs independent of the presence of the bacterium, but when the cagA pathogenicity marker is present, it induces changes in the gene expression of the respective miRNA.-
Descrição: dc.descriptionFaculdade de Medicina de Marília, SP-
Descrição: dc.descriptionFaculdade de Medicina de Botucatu, SP-
Descrição: dc.descriptionUniversidade Estadual de Campinas, SP-
Descrição: dc.descriptionUniversidade Federal de Goiás, GO-
Descrição: dc.descriptionUniversidade Federal de São Paulo, SP-
Descrição: dc.descriptionCentro Universitário das Faculdades Integradas de Ourinhos, SP-
Descrição: dc.descriptionFaculdade de Medicina de Botucatu, SP-
Idioma: dc.languageen-
Idioma: dc.languagept_BR-
Relação: dc.relationArquivos de Gastroenterologia-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectchronic gastritis-
Palavras-chave: dc.subjectgastric diseases-
Palavras-chave: dc.subjectHelicobacter pylori-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectmicroRNA-
Palavras-chave: dc.subjectvirulence factors-
Título: dc.titleDecreased expression of microRNA-629 in gastric cancer samples potentiated by the virulence marker of H. pylori, cagA gene-
Título: dc.titleDiminuição da expressão do microRNA-629 em amostras de câncer gástrico potencializada pelo marcador de virulências do H. pylori, gene cagA-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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