Expression of Interleukin-1β protein in vitro, ex vivo and in vivo salmonid models

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Autor(es): dc.contributorUniversity of Waterloo-
Autor(es): dc.contributorMemorial University of Newfoundland and Labrador-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCedarlane Laboratories-
Autor(es): dc.creatorFrenette, Aaron P.-
Autor(es): dc.creatorRodríguez-Ramos, Tania-
Autor(es): dc.creatorZanuzzo, Fabio-
Autor(es): dc.creatorRamsay, Devyn-
Autor(es): dc.creatorSemple, Shawna L.-
Autor(es): dc.creatorSoullière, Cheryl-
Autor(es): dc.creatorRodríguez-Cornejo, Tania-
Autor(es): dc.creatorHeath, George-
Autor(es): dc.creatorMcKenzie, Emily-
Autor(es): dc.creatorIwanczyk, Jack-
Autor(es): dc.creatorBruder, Mark-
Autor(es): dc.creatorAucoin, Marc G.-
Autor(es): dc.creatorGamperl, A. Kurt-
Autor(es): dc.creatorDixon, Brian-
Data de aceite: dc.date.accessioned2025-08-21T23:42:51Z-
Data de disponibilização: dc.date.available2025-08-21T23:42:51Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dci.2023.104767-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300974-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300974-
Descrição: dc.descriptionInterleukin-1β (IL-1β) is one of the first cytokines expressed during immune responses, and its levels are affected by many factors, including stress. To date, it has only been possible to measure IL-1β transcript (mRNA) expression quantitatively in fish using qPCR. This is because previous studies that measured IL-1β protein concentrations in these taxa used western blotting, which only provides qualitative data. To advance our knowledge of fish IL-1β biology, and because post-translational processing plays a critical role in the activation of this molecule, we developed a quantitative enzyme-linked immunosorbent assay (ELISA) to accurately measure the concentration of IL-1β protein in several cell cultures and in vivo in salmonids. We compared changes in IL-1β protein levels to the expression of its mRNA. The developed ELISA was quite sensitive and has a detection limit of 12.5 pg/mL. The tools developed, and information generated through this research, will allow for a more accurate and complete understanding of IL-1β′s role in the immune response of salmonids.The assay described here has the potential to significantly advance our ability to assess fish health and immune status.-
Descrição: dc.descriptionAtlantic Canada Opportunities Agency-
Descrição: dc.descriptionDepartment of Biology University of Waterloo-
Descrição: dc.descriptionDepartment of Ocean Sciences Memorial University of Newfoundland and Labrador-
Descrição: dc.descriptionUniversidade Estadual Paulista – UNESP Centro de Aquicultura da UNESP Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, SP-
Descrição: dc.descriptionCedarlane Laboratories-
Descrição: dc.descriptionDepartment of Chemical Engineering University of Waterloo-
Descrição: dc.descriptionUniversidade Estadual Paulista – UNESP Centro de Aquicultura da UNESP Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane, SP-
Descrição: dc.descriptionAtlantic Canada Opportunities Agency: 5404–1209-104-
Descrição: dc.descriptionAtlantic Canada Opportunities Agency: 781–9658-205222-
Idioma: dc.languageen-
Relação: dc.relationDevelopmental and Comparative Immunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell culture-
Palavras-chave: dc.subjectDiagnostic assay-
Palavras-chave: dc.subjectImmunostimulation-
Palavras-chave: dc.subjectInterleukin-1β-
Palavras-chave: dc.subjectNatural infection-
Palavras-chave: dc.subjectQuantitative ELISA-
Título: dc.titleExpression of Interleukin-1β protein in vitro, ex vivo and in vivo salmonid models-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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