Morin-Loaded Chitosan-Poloxamer Hydrogel as an Osteoinductive Delivery System for Endodontic Applications

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade do Porto-
Autor(es): dc.contributorUniversidade de Lisboa-
Autor(es): dc.contributorInstituto Politécnico de Setúbal-
Autor(es): dc.contributorUniversidade Católica Portuguesa (UCP)-
Autor(es): dc.creatorPereira, Jesse Augusto-
Autor(es): dc.creatorMartin, Victor-
Autor(es): dc.creatorAraújo, Rita-
Autor(es): dc.creatorGrenho, Liliana-
Autor(es): dc.creatorGomes, Pedro-
Autor(es): dc.creatorMarto, Joana-
Autor(es): dc.creatorFernandes, Maria Helena-
Autor(es): dc.creatorSantos, Catarina-
Autor(es): dc.creatorDuque, Cristiane-
Data de aceite: dc.date.accessioned2025-08-21T19:05:08Z-
Data de disponibilização: dc.date.available2025-08-21T19:05:08Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jbm.a.37895-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300969-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300969-
Descrição: dc.descriptionConsidering the search for new biocompatible intracanal medicaments that can preserve remaining cells and stimulate bone tissue repair in the periapical region, this study aimed to synthesize and characterize the physicochemical properties of morin-loaded chitosan-poloxamer hydrogel (MCP) as well as to evaluate its osteogenic potential. Morin hydrate (M) was loaded into chitosan-poloxamer (CP) hydrogel and the resulting particles were characterized by infrared spectroscopy (FTIR), UV–vis spectrophotometer and scanning electron microscopy. Biological assays evaluated the metabolic activity, cell morphology and alkaline phosphatase (ALP) activity of human bone marrow stem cells (HBMSC) in three different settings, such as the exposure to dissolved morin, hydrogel's leachates and assembled particles by indirect contact. Cells cultured in standard culture conditions were used as control. The effect of CP and MCP particles on the formation of collagenous and mineralized tissues was also assessed within the organotypic model of segmented embryonic chick femora. Datasets were assessed for one-way analysis of variance (ANOVA), followed by Tukey's post hoc test (p < 0.05). Morin at 50 μg/mL was cytocompatible and increased ALP activity. CP and MCP particles showed stability, and morin was entrapped in the hydrogel matrix without changing its chemical structure. Cultures treated with 30-min CP and MCP hydrogel leachates presented significantly higher metabolic activity compared to control. By indirect contact, CP particles increased metabolic activity, but only MCP particles induced an upregulation of ALP activity in comparison to control. The amount of collagenous tissue and mineralized area on the fractured embryonic chick femora was greater in MCP particles compared to CP counterparts. Chitosan-poloxamer platforms are suitable systems to delivery morin, enhancing cell proliferation and bone mineralization, which upholds its application as intracanal medication for endodontic purposes.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP)-
Descrição: dc.descriptionBoneLab Faculdade de Medicina Dentária Universidade do Porto-
Descrição: dc.descriptionLAQV/REQUIMTE Faculdade de Medicina Dentária Universidade do Porto-
Descrição: dc.descriptionResearch Institute for Medicines (iMed.ULisboa) Faculty of Pharmacy Universidade de Lisboa-
Descrição: dc.descriptionEST Setúbal CDP2T Instituto Politécnico de Setúbal-
Descrição: dc.descriptionCQE Instituto Superior Técnico Universidade de Lisboa-
Descrição: dc.descriptionFaculty of Dental Medicine Center for Interdisciplinary Research in Health (CIIS) Universidade Católica Portuguesa (UCP)-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry Araçatuba Dental School São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Biomedical Materials Research - Part A-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiocompatibility-
Palavras-chave: dc.subjectchitosan-
Palavras-chave: dc.subjectdrug-delivery-
Palavras-chave: dc.subjectMorin-
Palavras-chave: dc.subjectOsteogenesis-
Palavras-chave: dc.subjectPoloxamer-
Palavras-chave: dc.subjectstem cells-
Título: dc.titleMorin-Loaded Chitosan-Poloxamer Hydrogel as an Osteoinductive Delivery System for Endodontic Applications-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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