MicroRNA biogenesis machinery activation and lncRNA and REST overexpression as neuroprotective responses to fight inflammation in the hippocampus

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Taubaté-
Autor(es): dc.contributorPaulista University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de Santa Catarina (UFSC)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversity of Wisconsin-Madison-
Autor(es): dc.contributorColumbia University Medical Center-
Autor(es): dc.creatorCarvalho, Liebert Bernardes-
Autor(es): dc.creatordos Santos Sanna, Paula Lemes-
Autor(es): dc.creatordos Santos Afonso, Camila Cristina-
Autor(es): dc.creatorBondan, Eduardo F.-
Autor(es): dc.creatorda Silva Feltran, Geórgia-
Autor(es): dc.creatorFerreira, Marcel Rodrigues-
Autor(es): dc.creatorBirbrair, Alexander-
Autor(es): dc.creatorAndia, Denise Carleto-
Autor(es): dc.creatorLatini, Alexandra-
Autor(es): dc.creatorFoganholi da Silva, Rodrigo A.-
Data de aceite: dc.date.accessioned2025-08-21T19:11:14Z-
Data de disponibilização: dc.date.available2025-08-21T19:11:14Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-09-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jneuroim.2023.578149-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300960-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300960-
Descrição: dc.descriptionBrain Long non-coding RNA (lncRNA) and microRNAs (miRs) play essential roles in the regulation of several important biological processes, including neuronal activity, cognitive processes, neurogenesis, angiogenesis, and neuroinflammation. In this context, the transcriptional repressor, RE1 silencing transcription factor (Rest), acts regulating the expression of neuronal genes as well as of lncRNAs and multiple miRNAs in the central nervous system. Nevertheless, its role in neuroinflammation was less explored. Here, we demonstrate, using an in vivo model of neuroinflammation induced by i.p. injection of LPS (0.33 mg/kg), that neuroinflammation increases gene expression of pro-inflammatory cytokines concomitant with the native and truncated forms of Rest and of non-coding RNAs. Additionally, the increased expression of enzymes Drosha ribonuclease III) (Drosha), Exportin 5 (Xpo5) and Endoribonuclease dicer (Dicer), associated with high expression of neuroprotective miRs 22 and 132 are indicative that the activation of biogenesis of miRs in the hippocampal region is a Central Nervous System (CNS) protective mechanism for the deleterious effects of neuroinflammation. Our results indicate that positive regulation of Rest gene expression in the hippocampal region by neuroinflammation correlates directly with the expression of miRs 22 and 132 and inversely with miR 335. In parallel, the confirmation of the possible alignment between the lncRNAs with miR 335 by bioinformatics corroborates with the sponge effect of Hottip and Hotair hybridizing and inhibiting the pro-inflammatory action of miR 335. This suggests the existence of a possible correlation between the activation of miR biogenesis machinery with increased expression of the transcription factor Rest, contributing to neuroprotection.-
Descrição: dc.descriptionDentistry University of Taubaté, Taubaté, SP-
Descrição: dc.descriptionCEEpiRG - Center for Epigenetic Study and Genic Regulation Program in Environmental and Experimental Pathology Paulista University, SP-
Descrição: dc.descriptionLab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP – São Paulo State University, SP-
Descrição: dc.descriptionLaboratory of Bioenergetics and Oxidative Stress LABOX Department of Biochemistry Center for Biological Sciences Federal University of Santa Catarina-
Descrição: dc.descriptionSchool of Dentistry Health Science Institute Paulista University, São Paulo-
Descrição: dc.descriptionDepartment of Pathology Federal University of Minas Gerais, MG-
Descrição: dc.descriptionDepartment of Dermatology University of Wisconsin-Madison-
Descrição: dc.descriptionDepartment of Radiology Columbia University Medical Center-
Descrição: dc.descriptionMolecular Genetics and Bioinformatics Laboratory Experimental Research Unity Botucatu Medical School São Paulo State University-
Descrição: dc.descriptionLab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP – São Paulo State University, SP-
Descrição: dc.descriptionMolecular Genetics and Bioinformatics Laboratory Experimental Research Unity Botucatu Medical School São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationJournal of Neuroimmunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHippocampus-
Palavras-chave: dc.subjectHotair-
Palavras-chave: dc.subjectHottip-
Palavras-chave: dc.subjectlncRNA-
Palavras-chave: dc.subjectmiR-
Palavras-chave: dc.subjectNeuroinflammation-
Palavras-chave: dc.subjectNon-coding RNAs-
Palavras-chave: dc.subjectRest-
Título: dc.titleMicroRNA biogenesis machinery activation and lncRNA and REST overexpression as neuroprotective responses to fight inflammation in the hippocampus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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