In-vitro polymicrobial oral biofilm model represents clinical microbial profile and disease progression during implant-related infections

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Pittsburgh-
Autor(es): dc.contributorGuarulhos University-
Autor(es): dc.contributorHarvard School of Dental Medicine-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorDini, Caroline-
Autor(es): dc.creatorCosta, Raphael Cavalcante-
Autor(es): dc.creatorBertolini, Martinna-
Autor(es): dc.creatorShibli, Jamil Awad-
Autor(es): dc.creatorFeres, Magda-
Autor(es): dc.creatorKlein, Marlise Inêz-
Autor(es): dc.creatorde Avila, Érica Dorigatti-
Autor(es): dc.creatorSouza, João Gabriel Silva-
Autor(es): dc.creatorBarão, Valentim Adelino Ricardo-
Data de aceite: dc.date.accessioned2025-08-21T20:31:53Z-
Data de disponibilização: dc.date.available2025-08-21T20:31:53Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jambio/lxad265-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300882-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300882-
Descrição: dc.descriptionAim: Clinically relevant in-vitro biofilm models are essential and valuable tools for mechanistically dissecting the etiopathogenesis of infectious diseases and test new antimicrobial therapies. Thus, the aim of this study was to develop and test a clinically relevant in-vitro oral polymicrobial biofilm model that mimics implant-related infections in terms of microbial profile. Methods and Results: For this purpose, 24-well plate system was used to model oral biofilms, using three different microbial inoculums to grow in-vitro biofilms: (1) human saliva from periodontally healthy patients; (2) saliva as in inoculum 1 + Porphyromonas gingivalis strain; and (3) supra and subgingival biofilm collected from peri-implant sites of patients diagnosed with peri-implantitis. Biofilms were grown to represent the dynamic transition from an aerobic to anaerobic community profile. Subsequently, biofilms were collected after each phase and evaluated for microbiological composition, microbial counts, biofilm biomass, structure, and susceptibility to chlorhexidine (CHX). Results showed higher live cell count (P < .05) for biofilms developed from patients’ biofilm inoculum, but biomass volume, dry weight, and microbiological composition were similar among groups (P > .05). Interestingly, according to the checkerboard DNA–DNA hybridization results, the biofilm developed from stimulated human saliva exhibited a microbial composition more similar to the clinical subgingival biofilm of patients with peri-implantitis, with proportions of the main pathogens closer to those found in the disease. In addition, biofilm developed using saliva as inoculum was shown to be susceptible to CHX with significant reduction in bacteria compared with biofilms without exposure to CHX (P < .05). Conclusion: The findings suggested that the in-vitro polymicrobial biofilm developed from human saliva as inoculum is a suitable model and clinically relevant tool for mimicking the microbial composition of implant-related infections.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Prosthodontics and Periodontology Piracicaba Dental School Universidade Estadual de Campinas (UNICAMP), SP-
Descrição: dc.descriptionDepartment of Periodontics and Preventive Dentistry School of Dental Medicine University of Pittsburgh-
Descrição: dc.descriptionDental Research Division Guarulhos University, SP-
Descrição: dc.descriptionDepartment of Oral Medicine Infection and Immunity Harvard School of Dental Medicine-
Descrição: dc.descriptionDepartment of Oral Diagnosis Piracicaba Dental School Universidade Estadual de Campinas (UNICAMP), SP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araçatuba São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry at Araçatuba São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCNPq: #307471/2021-7-
Descrição: dc.descriptionCNPq: #311368/2019-0-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2018/20719-3-
Descrição: dc.descriptionFAPESP: 2020/05231-4-
Descrição: dc.descriptionFAPESP: 2020/05234-3-
Descrição: dc.descriptionFAPESP: 2021/09434-0-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectdental implants-
Palavras-chave: dc.subjectin-vitro models-
Palavras-chave: dc.subjectperi-implantitis-
Título: dc.titleIn-vitro polymicrobial oral biofilm model represents clinical microbial profile and disease progression during implant-related infections-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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