Biomechanically induced regulation of Damage-Regulated Autophagy Modulator 1 in periodontal cells and tissues

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Autor(es): dc.contributorUniversity of Bonn-
Autor(es): dc.contributorUniversity Medical Center of the Johannes Gutenberg University Mainz-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMannes, Anemone-
Autor(es): dc.creatorNogueira, Andressa-
Autor(es): dc.creatorBoth, Annika-
Autor(es): dc.creatorMayr, Alexandra-
Autor(es): dc.creatorMarciniak, Jana-
Autor(es): dc.creatorKüchler, Erika Calvano-
Autor(es): dc.creatorBekbulat, Fazilet-
Autor(es): dc.creatorCirelli, Joni A.-
Autor(es): dc.creatorKirschneck, Christian-
Autor(es): dc.creatorBehl, Christian-
Autor(es): dc.creatorDeschner, James-
Autor(es): dc.creatorJäger, Andreas-
Autor(es): dc.creatorBeisel-Memmert, Svenja-
Data de aceite: dc.date.accessioned2025-08-21T22:08:56Z-
Data de disponibilização: dc.date.available2025-08-21T22:08:56Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2024.151131-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300847-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300847-
Descrição: dc.descriptionPurpose: Autophagy is an important adaptive process for mechanotransduction, in which Damage-Regulated Autophagy Modulator 1 (DRAM1) has a key function in cell fate determination. This study aimed to analyze the influence of biomechanical loading on DRAM1 expression in periodontal cells and tissues. Methods: Human periodontal ligament (PDL) fibroblasts were stimulated with different pressure protocols, physiological load and overload, in the presence and absence of autophagy inhibitor 3-methyladenine (3-MA) and compared with untreated cells. DRAM1 expression was measured using real-time PCR and ELISA after 1 d and 2 d. DRAM1 expression was determined in gingival biopsies of rats, and gene expression of DRAM1 was analyzed after 1 d, 7 d, and 15 d of orthodontic treatment. Statistical analysis was carried out using ANOVA and post-hoc tests. Results: Overload led to increased DRAM1 gene expression after 1 d, while physiological load did not change DRAM1 expression. After 2 d, DRAM1 expression was increased in both groups. Protein levels were elevated after 2 d of pressure application of both magnitudes, while no significant increase was evident after 1 d. Treatment with 3-MA led to a significant reduction in DRAM1 gene expression in both pressure groups, while it remained unchanged in the control group. In vivo, DRAM1 was located in the periodontal ligament, and we could determine an orthodontic force-mediated increase in DRAM1 gene expression at 7 d and 15 d. Conclusion: This study indicates a dependence of DRAM1 regulation on the duration and magnitude of bio-mechanical loading and on autophagy-associated pathways.-
Descrição: dc.descriptionDeutsche Forschungsgemeinschaft-
Descrição: dc.descriptionRheinische Friedrich-Wilhelms-Universität Bonn-
Descrição: dc.descriptionCorona-Stiftung-
Descrição: dc.descriptionDepartment of Orthodontics University Hospital Medical Faculty University of Bonn, Welschnonnenstr. 17-
Descrição: dc.descriptionDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University Mainz-
Descrição: dc.descriptionInstitute of Pathobiochemistry The Autophagy Lab University Medical Center of the Johannes Gutenberg University Mainz-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University – UNESP-
Idioma: dc.languageen-
Relação: dc.relationBiochemical and Biophysical Research Communications-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAutophagy-
Palavras-chave: dc.subjectDamage-regulated autophagy modulator-
Palavras-chave: dc.subjectLoad-
Palavras-chave: dc.subjectOrthodontic force application-
Palavras-chave: dc.subjectOverload-
Palavras-chave: dc.subjectPeriodontal ligament-
Título: dc.titleBiomechanically induced regulation of Damage-Regulated Autophagy Modulator 1 in periodontal cells and tissues-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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