Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Michigan School of Dentistry-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Michigan-
Autor(es): dc.creatorReis-Prado, Alexandre Henrique dos-
Autor(es): dc.creatorRahimnejad, Maedeh-
Autor(es): dc.creatorDal-Fabbro, Renan-
Autor(es): dc.creatorToledo, Priscila Toninatto Alves de-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorOliveira, Pedro Henrique Chaves de-
Autor(es): dc.creatorFenno, J. Christopher-
Autor(es): dc.creatorCintra, Luciano Tavares Angelo-
Autor(es): dc.creatorBenetti, Francine-
Autor(es): dc.creatorBottino, Marco C.-
Data de aceite: dc.date.accessioned2025-08-21T22:08:56Z-
Data de disponibilização: dc.date.available2025-08-21T22:08:56Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bioactmat.2024.12.026-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300820-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300820-
Descrição: dc.descriptionInjectable biomaterials, such as thermosensitive chitosan (CH)-based hydrogels, present a highly translational potential in dentistry due to their minimally invasive application, adaptability to irregular defects/shapes, and ability to carry therapeutic drugs. This work explores the incorporation of azithromycin (AZI) into thermosensitive CH hydrogels for use as an intracanal medication in regenerative endodontic procedures (REPs). The morphological and chemical characteristics of the hydrogel were assessed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). The thermosensitivity, gelation kinetics, compressive strength, cytocompatibility, and antibacterial efficacy were evaluated according to well-established protocols. An in vivo model of periapical disease and evoked bleeding in rats' immature permanent teeth was performed to determine disinfection, tissue repair, and root formation. AZI was successfully incorporated into interconnected porous CH hydrogels, which retained their thermosensitivity. The mechanical and rheological findings indicated that adding AZI did not adversely affect the hydrogels’ strength and injectability. Incorporating 3 % and 5 % AZI into the hydrogels led to minimal cytotoxic effects compared to higher concentrations while enhancing the antibacterial response against endodontic bacteria. AZI-laden hydrogel significantly decreased E. faecalis biofilm compared to the controls. Regarding tissue response, the 3 % AZI-laden hydrogel improved mineralized tissue formation and vascularization compared to untreated teeth and those treated with double antibiotic paste. Our findings demonstrate that adding 3 % AZI into CH hydrogels ablates infection and supports neotissue formation in vivo when applied to a clinically relevant model of regenerative endodontics.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionColorado Humanities-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionNational Institute of Dental and Craniofacial Research-
Descrição: dc.descriptionNational Institutes of Health-
Descrição: dc.descriptionFURTHERMORE grants in publishing-
Descrição: dc.descriptionDepartment of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry-
Descrição: dc.descriptionDepartment of Restorative Dentistry Universidade Federal de Minas Gerais (UFMG) School of Dentistry, MG-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Morphology and Pediatric Dentistry São Paulo State University (UNESP) - Araraquara School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Biologic and Materials Sciences & Prosthodontics University of Michigan School of Dentistry-
Descrição: dc.descriptionDepartment of Biomedical Engineering College of Engineering University of Michigan-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Morphology and Pediatric Dentistry São Paulo State University (UNESP) - Araraquara School of Dentistry, SP-
Formato: dc.format406-422-
Idioma: dc.languageen-
Relação: dc.relationBioactive Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibiotics-
Palavras-chave: dc.subjectAzithromycin-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectHydrogels-
Palavras-chave: dc.subjectInjectable-
Palavras-chave: dc.subjectRegenerative endodontics-
Título: dc.titleInjectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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