Antimicrobial peptides grafted onto the surface of N-acetylcysteine-chitosan nanoparticles can revitalize drugs against clinical isolates of Mycobacterium tuberculosis

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidad Católica de Santa María-
Autor(es): dc.contributorUniversity of Araraquara (UNIARA)-
Autor(es): dc.contributorUniversity of Copenhagen-
Autor(es): dc.creatorPrimo, Laura Maria Duran Gleriani-
Autor(es): dc.creatorRoque-Borda, Cesar Augusto-
Autor(es): dc.creatorCarnero Canales, Christian Shleider-
Autor(es): dc.creatorCaruso, Icaro Putinhon-
Autor(es): dc.creatorde Lourenço, Isabella Ottenio-
Autor(es): dc.creatorColturato, Vitória Maria Medalha-
Autor(es): dc.creatorSábio, Rafael Miguel-
Autor(es): dc.creatorde Melo, Fernando Alves-
Autor(es): dc.creatorVicente, Eduardo Festozo-
Autor(es): dc.creatorChorilli, Marlus-
Autor(es): dc.creatorda Silva Barud, Hernane-
Autor(es): dc.creatorBarbugli, Paula Aboud-
Autor(es): dc.creatorFranzyk, Henrik-
Autor(es): dc.creatorHansen, Paul Robert-
Autor(es): dc.creatorPavan, Fernando Rogério-
Data de aceite: dc.date.accessioned2025-08-21T18:22:41Z-
Data de disponibilização: dc.date.available2025-08-21T18:22:41Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.carbpol.2023.121449-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300709-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300709-
Descrição: dc.descriptionTuberculosis is caused by Mycobacterium tuberculosis (MTB) and is the leading cause of death from infectious diseases in the World. The search for new antituberculosis drugs is a high priority, since several drug-resistant TB-strains have emerged. Many nanotechnology strategies are being explored to repurpose or revive drugs. An interesting approach is to graft antimicrobial peptides (AMPs) to antibiotic-loaded nanoparticles. The objective of the present work was to determine the anti-MTB activity of rifampicin-loaded N-acetylcysteine-chitosan-based nanoparticles (NPs), conjugated with the AMP Ctx(Ile21)-Ha; against clinical isolates (multi- and extensively-drug resistant) and the H37Rv strain. The modified chitosan and drug-loaded NPs were characterized with respect to their physicochemical stability and their antimycobacterial profile, which showed potent inhibition (MIC values <0.977 μg/mL) by the latter. Furthermore, their accumulation within macrophages and cytotoxicity were determined. To understand the possible mechanisms of action, an in silico study of the peptide against MTB membrane receptors was performed. The results presented herein demonstrate that antibiotic-loaded NPs grafted with an AMP can be a powerful tool for revitalizing drugs against multidrug-resistant M. tuberculosis strains, by launching multiple attacks against MTB. This approach could potentially serve as a novel treatment strategy for various long-term diseases requiring extended treatment periods.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Tuberculosis Research Laboratory School of Pharmaceutical Sciences, São Paulo-
Descrição: dc.descriptionVicerrectorado de Investigación Facultad de Ciencias Farmacéuticas bioquímicas y biotecnológicas Universidad Católica de Santa María-
Descrição: dc.descriptionDepartment of Physics - Institute of Biosciences Humanities and Exact Sciences (IBILCE) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Biotechnology Laboratory of Polymers and Biomaterials University of Araraquara (UNIARA), São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Drug and Medicines School of Pharmaceutical Sciences, São Paulo-
Descrição: dc.descriptionSchool of Sciences and Engineering São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry Sao Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Drug Design and Pharmacology Faculty of Health and Medical Sciences University of Copenhagen-
Descrição: dc.descriptionSão Paulo State University (UNESP) Tuberculosis Research Laboratory School of Pharmaceutical Sciences, São Paulo-
Descrição: dc.descriptionDepartment of Physics - Institute of Biosciences Humanities and Exact Sciences (IBILCE) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Drug and Medicines School of Pharmaceutical Sciences, São Paulo-
Descrição: dc.descriptionSchool of Sciences and Engineering São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry Sao Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionFAPESP: 2013/07276-1-
Descrição: dc.descriptionFAPESP: 2016/00446-7-
Descrição: dc.descriptionFAPESP: 2020/13497-4-
Descrição: dc.descriptionFAPESP: 2020/16573-3-
Descrição: dc.descriptionFAPESP: 2021/14603-5-
Descrição: dc.descriptionFAPESP: 2022/09728-6-
Descrição: dc.descriptionFAPESP: 2023/01664-1-
Descrição: dc.descriptionCNPq: 303603/2018-6-
Descrição: dc.descriptionCNPq: 309614/2021-0-
Descrição: dc.descriptionCNPq: 404181/2019-8-
Descrição: dc.descriptionCNPq: 429139/2018-7-
Idioma: dc.languageen-
Relação: dc.relationCarbohydrate Polymers-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial peptide-
Palavras-chave: dc.subjectExtensively drug-resistant-
Palavras-chave: dc.subjectMycobacterium tuberculosis-
Palavras-chave: dc.subjectN-acetylcysteine-chitosan-
Título: dc.titleAntimicrobial peptides grafted onto the surface of N-acetylcysteine-chitosan nanoparticles can revitalize drugs against clinical isolates of Mycobacterium tuberculosis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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