Exploring Canine Mammary Cancer through Liquid Biopsy: Proteomic Profiling of Small Extracellular Vesicles

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de Mato Grosso (UFMT)-
Autor(es): dc.contributorBrazilian Center for Research in Energy and Materials (CNPEM)-
Autor(es): dc.contributorFaculdade de Medicina de São José do Rio Preto/(FAMERP)-
Autor(es): dc.contributorPat Animal Laboratory-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorOhio State University-
Autor(es): dc.creatorNovais, Adriana Alonso-
Autor(es): dc.creatorTamarindo, Guilherme Henrique-
Autor(es): dc.creatorMelo, Luryan Mikaelly Minotti-
Autor(es): dc.creatorBalieiro, Beatriz Castilho-
Autor(es): dc.creatorNóbrega, Daniela-
Autor(es): dc.creatordos Santos, Gislaine-
Autor(es): dc.creatorSaldanha, Schaienni Fontoura-
Autor(es): dc.creatorde Souza, Fabiana Ferreira-
Autor(es): dc.creatorChuffa, Luiz Gustavo de Almeida-
Autor(es): dc.creatorBracha, Shay-
Autor(es): dc.creatorZuccari, Debora Aparecida Pires de Campos-
Data de aceite: dc.date.accessioned2025-08-21T17:32:54Z-
Data de disponibilização: dc.date.available2025-08-21T17:32:54Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/cancers16142562-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300632-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300632-
Descrição: dc.description(Background). Canine mammary tumors (CMTs) have emerged as an important model for understanding pathophysiological aspects of human disease. Liquid biopsy (LB), which relies on blood-borne biomarkers and offers minimal invasiveness, holds promise for reflecting the disease status of patients. Small extracellular vesicles (SEVs) and their protein cargo have recently gained attention as potential tools for disease screening and monitoring. (Objectives). This study aimed to isolate SEVs from canine patients and analyze their proteomic profile to assess their diagnostic and prognostic potential. (Methods). Plasma samples were collected from female dogs grouped into CMT (malignant and benign), healthy controls, relapse, and remission groups. SEVs were isolated and characterized using ultracentrifugation (UC), nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM). Proteomic analysis of circulating SEVs was conducted using liquid chromatography–mass spectrometry (LC–MS). (Results). While no significant differences were observed in the concentration and size of exosomes among the studied groups, proteomic profiling revealed important variations. Mass spectrometry identified exclusive proteins that could serve as potential biomarkers for mammary cancer. These included Inter-alpha-trypsin inhibitor heavy chain (ITIH2 and ITI4), phosphopyruvate hydratase or alpha enolase (ENO1), eukaryotic translation elongation factor 2 (eEF2), actin (ACTB), transthyretin (TTR), beta-2-glycoprotein 1 (APOH) and gelsolin (GSN) found in female dogs with malignant tumors. Additionally, vitamin D-binding protein (VDBP), also known as group-specific component (GC), was identified as a protein present during remission. (Conclusions). The results underscore the potential of proteins found in SEVs as valuable biomarkers in CMTs. Despite the lack of differences in vesicle concentration and size between the groups, the analysis of protein content revealed promising markers with potential applications in CMT diagnosis and monitoring. These findings suggest a novel approach in the development of more precise and effective diagnostic tools for this challenging clinical condition.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstitute of Health Science (ICS) Universidade Federal de Mato Grosso (UFMT), MT-
Descrição: dc.descriptionBrazilian Biosciences National Laboratory Brazilian Center for Research in Energy and Materials (CNPEM), SP-
Descrição: dc.descriptionMolecular Investigation of Cancer Laboratory (MICL) Department of Molecular Biology Faculdade de Medicina de São José do Rio Preto/(FAMERP), SP-
Descrição: dc.descriptionPat Animal Laboratory, SP-
Descrição: dc.descriptionLaboratory of Molecular Morphophysiology and Development (LMMD/ZMV) University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science FMVZ São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP—São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Veterinary Clinical Sciences College of Veterinary Medicine Ohio State University-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science FMVZ São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences UNESP—São Paulo State University, SP-
Descrição: dc.descriptionFAPESP: 2020/12970-8-
Idioma: dc.languageen-
Relação: dc.relationCancers-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiomarker-
Palavras-chave: dc.subjectcanine-
Palavras-chave: dc.subjectdiagnosis-
Palavras-chave: dc.subjectliquid biopsy-
Palavras-chave: dc.subjectmammary carcinoma-
Palavras-chave: dc.subjectproteomics-
Palavras-chave: dc.subjectsmall extracellular vesicles-
Título: dc.titleExploring Canine Mammary Cancer through Liquid Biopsy: Proteomic Profiling of Small Extracellular Vesicles-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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