Chitosan-coated nanoemulsion for intranasal administration increases temozolomide mucosal permeation, cellular uptake, and In vitro cytotoxicity in glioblastoma multiforme cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorDuarte, Jonatas Lobato-
Autor(es): dc.creatorDi Filippo, Leonardo Delello-
Autor(es): dc.creatorAzevedo Vilella, Kelle Jarcy-
Autor(es): dc.creatorPaes Dutra, Jessyca Aparecida-
Autor(es): dc.creatorRibeiro, Diego Messalle-
Autor(es): dc.creatorFreitas da Silva, Mônica-
Autor(es): dc.creatorIvo de Medeiros, Alexandra-
Autor(es): dc.creatorChorilli, Marlus-
Data de aceite: dc.date.accessioned2025-08-21T15:46:57Z-
Data de disponibilização: dc.date.available2025-08-21T15:46:57Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jddst.2024.106390-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300611-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300611-
Descrição: dc.descriptionGlioblastoma multiforme (GBM) is the most prevalent and aggressive type of brain cancer in adults. Temozolomide (TMZ) is the chemotherapeutic agent used to treat primary central nervous system tumors. However, TMZ's clinical effectiveness faces several challenges due to its physical-chemical properties and biological features of GBM, such as the blood-brain barrier (BBB). Mucoadhesive nanosystems such as those coated with chitosan represent a promising alternative for optimizing the delivery of therapeutic agents to the central nervous system, as they possess ideal characteristics that enhance their interaction with the intranasal mucosa. We aimed to develop a chitosan-coated nanoemulsion containing temozolomide (CS-NE-TMZ) for nose-to-brain delivery and characterize its physical-chemical and in vitro biological properties. CS-NE-TMZ were obtained by emulsification followed by sonication. The optimized CS-NE-TMZ presented droplet size of 123,4 ± 2,3 nm, polydispersity index of 0.273 ± 0.028, zeta potential of +21,5 ± 0,81 mV, entrapment efficiency of 100 ± 1,91 % and drug loading of 2 ± 0,007 %. An in vitro release study of CS-NE-TMZ showed sustained release for up to 24 h following the Korsmeyer-Peppas model with Fickian diffusion. CS-NE-TMZ demonstrated significantly enhanced ex vivo mucosal permeation, compared to free TMZ, and showed enhanced in vitro cellular uptake, selectively increasing cytotoxicity in U-87MG glioma cells but not in healthy L929 fibroblasts, reinforcing the potential of mucoadhesive nanoemulsions as effective intranasal drug delivery systems for future brain cancer therapies.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionInstituto de Química de São Carlos Departamento de físico-química Universidade de São Paulo (USP), São Carlos-
Descrição: dc.descriptionDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara-
Descrição: dc.descriptionFAPESP: #2014/50928-2-
Descrição: dc.descriptionFAPESP: #2020/12622-0-
Descrição: dc.descriptionFAPESP: #2021/02609-9-
Descrição: dc.descriptionFAPESP: #2022/11101-1-
Descrição: dc.descriptionCNPq: #465687/2014-8-
Idioma: dc.languageen-
Relação: dc.relationJournal of Drug Delivery Science and Technology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrain cancer-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectDrug delivery-
Palavras-chave: dc.subjectLipid nanocarrier-
Palavras-chave: dc.subjectNose-to-brain-
Título: dc.titleChitosan-coated nanoemulsion for intranasal administration increases temozolomide mucosal permeation, cellular uptake, and In vitro cytotoxicity in glioblastoma multiforme cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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