Genomic Analysis of a Community-Acquired Methicillin-Resistant Staphylococcus aureus Sequence Type 1 Associated with Caprine Mastitis

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Autor(es): dc.contributorUniversidade Federal da Paraíba (UFPB)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorNational Institute of Semiarid (INSA)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorThe University of North Carolina at Charlotte-
Autor(es): dc.creatorVasconcelos, Priscylla C.-
Autor(es): dc.creatorLeite, Elma L.-
Autor(es): dc.creatorSaraiva, Mauro M. S.-
Autor(es): dc.creatorFerrari, Rafaela G.-
Autor(es): dc.creatorCibulski, Samuel P.-
Autor(es): dc.creatorSilva, Nubia M. V.-
Autor(es): dc.creatorFreitas Neto, Oliveiro C.-
Autor(es): dc.creatorGivisiez, Patrícia E. N.-
Autor(es): dc.creatorVieira, Rafael F. C.-
Autor(es): dc.creatorOliveira, Celso J. B.-
Data de aceite: dc.date.accessioned2025-08-21T17:15:48Z-
Data de disponibilização: dc.date.available2025-08-21T17:15:48Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pathogens13010023-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300608-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300608-
Descrição: dc.descriptionThis study aimed to investigate the genomic and epidemiological features of a methicillin-resistant Staphylococcus aureus sequence type 1 (MRSA ST1) strain associated with caprine subclinical mastitis. An S. aureus strain was isolated from goat’s milk with subclinical mastitis in Paraiba, Northeastern Brazil, by means of aseptic procedures and tested for antimicrobial susceptibility using the disk-diffusion method. Whole genome sequencing was performed using the Illumina MiSeq platform. After genome assembly and annotation, in silico analyses, including multilocus sequence typing (MLST), antimicrobial resistance and stress-response genes, virulence factors, and plasmids detection were performed. A comparative SNP-based phylogenetic analysis was performed using publicly available MRSA genomes. The strain showed phenotypic resistance to cefoxitin, penicillin, and tetracycline and was identified as sequence type 1 (ST1) and spa type 128 (t128). It harbored the SCCmec type IVa (2B), as well as the lukF-PV and lukS-PV genes. The strain was phylogenetically related to six community-acquired MRSA isolates (CA-MRSA) strains associated with human clinical disease in North America, Europe, and Australia. This is the first report of a CA-MRSA strain associated with milk in the Americas. The structural and epidemiologic features reported in the MRSA ST1 carrying a mecA-SCCmec type IVa suggest highly complex mechanisms of horizontal gene transfer in MRSA. The SNP-based phylogenetic analysis suggests a zooanthroponotic transmission, i.e., a strain of human origin.-
Descrição: dc.descriptionDepartment of Animal Science College for Agricultural Sciences Federal University of Paraiba (CCA/UFPB), PB-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences Department of Pathology Reproduction and One Health São Paulo State University (Unesp), SP-
Descrição: dc.descriptionCenter for Biotechnology (CBiotec) Federal University of Paraiba (CBiotec/UFPB), PB-
Descrição: dc.descriptionAnimal Production Center National Institute of Semiarid (INSA), PB-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine Veterinary School Federal University of Minas Gerais (UFMG), MG-
Descrição: dc.descriptionDepartment of Public Health Sciences The University of North Carolina at Charlotte-
Descrição: dc.descriptionCenter for Computational Intelligence to Predict Health and Environmental Risks (CIPHER) The University of North Carolina at Charlotte-
Descrição: dc.descriptionSchool of Agricultural and Veterinarian Sciences Department of Pathology Reproduction and One Health São Paulo State University (Unesp), SP-
Idioma: dc.languageen-
Relação: dc.relationPathogens-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantimicrobial resistance genes-
Palavras-chave: dc.subjectgoat milk-
Palavras-chave: dc.subjectmecA-
Palavras-chave: dc.subjectMRSA-
Palavras-chave: dc.subjectplasmids-
Palavras-chave: dc.subjectvirulence genes-
Palavras-chave: dc.subjectwhole genome sequencing-
Título: dc.titleGenomic Analysis of a Community-Acquired Methicillin-Resistant Staphylococcus aureus Sequence Type 1 Associated with Caprine Mastitis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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