Comparing the wound healing potential of natural rubber latex serum and F1-protein: An in vivo approach

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Autor(es): dc.contributorFederal University of Triangulo Mineiro (UFTM)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorImperial College London-
Autor(es): dc.contributorPontificia Universidad Católica de Valparaíso-
Autor(es): dc.contributorUniversity Center of Hermínio Ometto Foundation (FHO)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorTerasaki Institute for Biomedical Innovation (TIBI)-
Autor(es): dc.contributorCalifornia State University-
Autor(es): dc.creatorMorais, Pamela Cássia Rocha-
Autor(es): dc.creatorFloriano, Juliana Ferreira-
Autor(es): dc.creatorGarcia, Cristiane Garcia Paulino-
Autor(es): dc.creatorChagas, Ana Laura Destro-
Autor(es): dc.creatorMussagy, Cassamo Ussemane-
Autor(es): dc.creatorGuerra, Nayrim Brizuela-
Autor(es): dc.creatorSant'Ana Pegorin Brasil, Giovana-
Autor(es): dc.creatorVicentine, Karina Ferrazzoli Devienne-
Autor(es): dc.creatorRocha, Lenaldo Branco-
Autor(es): dc.creatorOliveira, Carlo José Freire-
Autor(es): dc.creatorSoares de Oliveira Junior, Robson Tadeu-
Autor(es): dc.creatorCaetano, Guilherme Ferreira-
Autor(es): dc.creatorLi, Bingbing-
Autor(es): dc.creatordos Santos, Lindomar Soares-
Autor(es): dc.creatorHerculano, Rondinelli Donizetti-
Autor(es): dc.creatorde Mendonça, Ricardo José-
Data de aceite: dc.date.accessioned2025-08-21T18:58:31Z-
Data de disponibilização: dc.date.available2025-08-21T18:58:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bioadv.2023.213754-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300560-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300560-
Descrição: dc.descriptionChronic wounds pose significant health concerns. Current treatment options include natural compounds like natural rubber latex (NRL) from Hevea brasiliensis. NRL, particularly the F1 protein fraction, has demonstrated bioactivity, biocompatibility, and angiogenic effects. So far, there is no study comparing F1 protein with total NRL serum, and the necessity of downstream processing remains unknown. Here, we evaluated the angiogenic potential of F1 protein compared to total NRL serum and the need for downstream processing. For that, ion exchange chromatography (DEAE-Sepharose), antioxidant activity, physicochemical characterization, cell culture in McCoy fibroblasts, and wound healing in Balb-C mice were performed. Also, the evaluation of histology and collagen content and the levels of inflammatory mediators were quantified. McCoy fibroblast cell assay showed that F1 protein (0.01 %) and total NRL serum (0.01 %) significantly increased cell proliferation by 47.1 ± 11.3 % and 25.5 ± 2.5 %, respectively. However, the AA of F1 protein (78.9 ± 0.8 %) did not show a significant difference compared to NRL serum (77.0 ± 1.1 %). F1 protein and NRL serum were more effective in wound management in rodents. Histopathological analysis confirmed accelerated healing and advanced tissue repair. Similarly, the F1 protein (0.01 %) increased collagen, showing that this fraction can stimulate the synthesis of collagen by fibroblastic cells. Regarding cytokines production (IL-10, TNF-α, IFN-γ), F1 protein and NRL serum did not exert an impact on the synthesis of these cytokines. Furthermore, we did not observe statistically significant changes in dosages of enzymes (MPO and EPO) among the groups. Nevertheless, Nitric Oxide dosage was reduced drastically when the F1 protein (0.01 %) protein was applied topically. These findings contribute to the understanding of F1 protein and NRL serum properties and provide insights into cost-effectiveness and practical applications in medicine and biotechnology. Therefore, further research is needed to assess the economic feasibility of downstream processing for NRL-based herbal medicine derived from Hevea brasiliensis.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionPró-Reitoria de Pesquisa, Universidade Federal do Rio Grande do Sul-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Biochemistry Pharmacology and Physiology Federal University of Triangulo Mineiro (UFTM), Minas Gerais-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School, São Paulo-
Descrição: dc.descriptionNational Heart and Lung Institute Imperial College London-
Descrição: dc.descriptionBioengineering & Biomaterials Group São Paulo State University (UNESP) School of Pharmaceutical Sciences, Km 01 Araraquara-Jaú Road, São Paulo-
Descrição: dc.descriptionScience Faculty São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionEscuela de Agronomía Facultad de Ciencias Agronómicas y de los Alimentos Pontificia Universidad Católica de Valparaíso-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Pathology Genetics and Evolution Federal University of Triangulo Mineiro (UFTM), Minas Gerais-
Descrição: dc.descriptionDepartment of Microbiology Immunology and Parasitology Federal University of Triangulo Mineiro (UFTM), Minas Gerais-
Descrição: dc.descriptionGraduate Program of Orthodontics University Center of Hermínio Ometto Foundation (FHO), SP-
Descrição: dc.descriptionDivision of Dermatology Department of Internal Medicine University of São Paulo (USP) Ribeirão Preto Medical School, SP-
Descrição: dc.descriptionTerasaki Institute for Biomedical Innovation (TIBI), 11507 West Olympic Blvd-
Descrição: dc.descriptionAutonomy Research Center for STEAHM (ARCS) California State University-
Descrição: dc.descriptionFaculty of Philosophy Sciences and Languages at Ribeirão Preto University of São Paulo (USP), 3900 Bandeirantes Avenue, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School, São Paulo-
Descrição: dc.descriptionBioengineering & Biomaterials Group São Paulo State University (UNESP) School of Pharmaceutical Sciences, Km 01 Araraquara-Jaú Road, São Paulo-
Descrição: dc.descriptionScience Faculty São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionPró-Reitoria de Pesquisa, Universidade Federal do Rio Grande do Sul: 13/2022-
Descrição: dc.descriptionFAPESP: 2018/07342-8-
Descrição: dc.descriptionFAPESP: 2021/09207-3-
Descrição: dc.descriptionCNPq: 317203/2021-5-
Idioma: dc.languageen-
Relação: dc.relationBiomaterials Advances-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAngiogenic potential-
Palavras-chave: dc.subjectChronic wounds-
Palavras-chave: dc.subjectDownstream processing-
Palavras-chave: dc.subjectF1 protein-
Palavras-chave: dc.subjectNatural rubber latex-
Título: dc.titleComparing the wound healing potential of natural rubber latex serum and F1-protein: An in vivo approach-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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