Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorAssunpção Nishio, Suelen-
Autor(es): dc.creatorMoroz, Andrei-
Autor(es): dc.creatorde Assis Golim, Marjorie-
Autor(es): dc.creatorBraz, Aline Marcia Marques-
Autor(es): dc.creatorYokomichi, Anna Laura Yuri-
Autor(es): dc.creatorPedrosa, Valber A.-
Autor(es): dc.creatorCesarino, Ivana-
Autor(es): dc.creatorOliveira Jr., Osvaldo N.-
Autor(es): dc.creatorRibeiro, Sidney J.L.-
Autor(es): dc.creatorDeffune, Elenice-
Autor(es): dc.creatorMoraes, Marli Leite de-
Data de aceite: dc.date.accessioned2025-08-21T23:29:45Z-
Data de disponibilização: dc.date.available2025-08-21T23:29:45Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bioelechem.2025.108983-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300547-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300547-
Descrição: dc.descriptionFetomaternal hemorrhage, characterized by the passage of fetal blood into maternal circulation, poses significant risks to both mother and fetus. As it may be diagnosed by quantifying fetal red blood cells in maternal circulation, we developed an immunosensor to detect fetal cells made with a layer-by-layer silk fibroin film and a commercial CD71 monoclonal antibody. Detection was achieved through electrical impedance spectroscopy, allowing discrimination between human umbilical cord blood (UCB) cells and adult peripheral blood (APB) cells from patients and volunteers. Positive samples (UCB) exhibited an average capacitance of 0.34 nF at 1 to 10 Hz, while negative samples (APB) had 0.05 nF. Full agreement was obtained with the detection of fetal red blood cells using flow cytometry. The change in the electrical response was associated with the adsorption of UCB cells on the immunosensor, primarily due to conformational changes in the amide I groups. This reflects the immunoaffinity interactions, as revealed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Cell adsorption was confirmed with optical microscopy images, which indicate possible point-of-care strategies to diagnose FMH with image analysis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Medical School, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Clinical Hospital of Botucatu Medical School, SP-
Descrição: dc.descriptionFederal University of São Paulo (UNIFESP) Institute of Science and Technology, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Agricultural Sciences, SP-
Descrição: dc.descriptionUniversity of Sao Paulo (USP) Sao Carlos Institute of Physics, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Medical School, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Clinical Hospital of Botucatu Medical School, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Agricultural Sciences, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry, SP-
Descrição: dc.descriptionFAPESP: 2018/22214-6-
Idioma: dc.languageen-
Relação: dc.relationBioelectrochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCD71-
Palavras-chave: dc.subjectElectrical impedance spectroscopy-
Palavras-chave: dc.subjectFetomaternal hemorrhage-
Palavras-chave: dc.subjectGold interdigitated electrode-
Palavras-chave: dc.subjectImmunosensor-
Palavras-chave: dc.subjectSilk fibroin-
Título: dc.titleElectrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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