Automated impedance-based and manual leukocyte differential counts in healthy equines

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Autor(es): dc.contributorUniversity Center of the Integrated Faculties of Ourinhos (Unifio)-
Autor(es): dc.contributorOurinhos Veterinary Hospital (HVO)-
Autor(es): dc.contributorCanguru Veterinary Hospital-
Autor(es): dc.contributorAutonomous Veterinary-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorda Silva, Maria Fernanda Cazini-
Autor(es): dc.creatorde Brito, Luma Maria Rovina-
Autor(es): dc.creatorMartins, Beatriz de Azevedo-
Autor(es): dc.creatorLopes, Laryssa Eduarda de Campos-
Autor(es): dc.creatorDuarte, Yasmin Cunha-
Autor(es): dc.creatorde Morais, Matheus Nogueira-
Autor(es): dc.creatorVasconcelos, Laura Arduino-
Autor(es): dc.creatorMendonça, Mariana Orlandini-
Autor(es): dc.creatorMartins, Tainara de Oliveira-
Autor(es): dc.creatorMontechiesi, Daniela Fernandez-
Autor(es): dc.creatorde Almeida, Breno Fernando Martins-
Data de aceite: dc.date.accessioned2025-08-21T17:16:17Z-
Data de disponibilização: dc.date.available2025-08-21T17:16:17Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-27-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.26605/medvet-v17n4-5897-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300521-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300521-
Descrição: dc.descriptionThe speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count by impedance using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. All optical microscopy counts were carried out by the same veterinary clinical pathologist with ten years of previous experience in the area. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was-10.26% for segmented neutrophils, 11.04% for lymphocytes,-41.39% for monocytes, and-10.84% for eosinophils, ranging from-200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.-
Descrição: dc.descriptionDepartment of Veterinary Medicine Roque Quagliato Veterinary Hospital University Center of the Integrated Faculties of Ourinhos (Unifio), SP-
Descrição: dc.descriptionOurinhos Veterinary Hospital (HVO), SP-
Descrição: dc.descriptionCanguru Veterinary Hospital, SP-
Descrição: dc.descriptionAutonomous Veterinary, SP-
Descrição: dc.descriptionFaculty of Veterinary Medicine of Araçatuba São Paulo State University (FMVA-Unesp), SP-
Descrição: dc.descriptionFaculty of Veterinary Medicine of Araçatuba São Paulo State University (FMVA-Unesp), SP-
Formato: dc.format249-256-
Idioma: dc.languageen-
Relação: dc.relationMedicina Veterinaria (Brazil)-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHorse-
Palavras-chave: dc.subjectimpedance-
Palavras-chave: dc.subjectleukogram-
Título: dc.titleAutomated impedance-based and manual leukocyte differential counts in healthy equines-
Título: dc.titleContagem diferencial de leucócitos automatizada por impedância e manual em equinos saudáveis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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