Genetic characterization of lactococcosis-causing bacteria isolated from Brazilian native fish species

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Autor(es): dc.contributorNilton Lins University-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorAquaculture Research Center-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorBarbanti, Angelo Carlo Chaparro-
Autor(es): dc.creatordo Rosário, Angélica Emanuely Costa-
Autor(es): dc.creatorda Silva Maia, Cynthia Rafaela Monteiro-
Autor(es): dc.creatorRocha, Victória Pontes-
Autor(es): dc.creatorCosta, Henrique Lopes-
Autor(es): dc.creatorTrindade, Júlia Miranda-
Autor(es): dc.creatorNogueira, Luiz Fagner Ferreira-
Autor(es): dc.creatorRosa, Júlio César Câmara-
Autor(es): dc.creatorRanzani-Paiva, Maria José Tavares-
Autor(es): dc.creatorPilarski, Fabiana-
Autor(es): dc.creatorGallani, Silvia Umeda-
Autor(es): dc.creatorLeal, Carlos Augusto Gomes-
Autor(es): dc.creatorFigueiredo, Henrique César Pereira-
Autor(es): dc.creatorTavares, Guilherme Campos-
Data de aceite: dc.date.accessioned2025-08-21T17:12:35Z-
Data de disponibilização: dc.date.available2025-08-21T17:12:35Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-14-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.aquaculture.2024.741305-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300211-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300211-
Descrição: dc.descriptionPiscine lactococcosis is a disease caused by Lactococcus garvieae, L. petauri and L. formosensis. It is considered to be an emerging disease and poses a risk to global aquaculture production. Studies of the genetic diversity of Lactococcus spp. strains have epidemiological importance since they provide information on the genetic relationships between isolates and indicate geographic and temporal distribution. The aim of this study was to evaluate the genetic diversity of Brazilian Lactococcus spp. isolates obtained from native fish species using PCR-based DNA fingerprinting techniques. For this, 36 isolates were evaluated by gyrB sequencing, molecular serotyping, REP-, BOX-, and RAPD-PCR approaches. The discriminatory power and the congruence between the typing methods were calculated. After the correct taxonomic identification of the isolate, a total of 14, 5 and 17 isolates were reclassified as L. garvieae, L. formosensis and L. petauri, respectively. Lactococcus garvieae and L. petauri are related to serotype I, while L. formosensis strains were classified as serotype II. The typing methods tested are useful for evaluating the genetic diversity of isolates and a comprehensive indication of the diversity was found. Heterogeneous and homogeneous populations were observed for L. garvieae and L. petauri, respectively. Among the genotyping methods evaluated, RAPD-PCR demonstrated a greater discriminatory power for L. formosensis and L. petauri, while REP-PCR showed better results for L. garvieae strains, thus these methodologies are recommended for genotyping these bacterial species. In conclusion, Brazilian isolates of Lactococcus spp. obtained from native fish species constitute a genetically diverse population and the techniques used in this study allowed a better genetic characterization of the isolates.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Amazonas-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionPostgraduate Program in Aquaculture Nilton Lins University, Amazonas-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine School of Veterinary Medicine Federal University of Minas Gerais – UFMG, Minas Gerais-
Descrição: dc.descriptionFisheries Institute/APTA/SAA Aquaculture Research Center, São Paulo-
Descrição: dc.descriptionLaboratory of Microbiology and Parasitology of Aquatic Organisms São Paulo State University (Unesp) Aquaculture Center of Unesp, São Paulo-
Descrição: dc.descriptionLaboratory of Microbiology and Parasitology of Aquatic Organisms São Paulo State University (Unesp) Aquaculture Center of Unesp, São Paulo-
Descrição: dc.descriptionFAPEMIG: 01.02.016301.03071/2022-11-
Descrição: dc.descriptionFAPEMIG: APQ-01227-22-
Descrição: dc.descriptionFAPEMIG: APQ-04309-22-
Descrição: dc.descriptionFAPEMIG: PPM-00779-18-
Idioma: dc.languageen-
Relação: dc.relationAquaculture-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectGenotyping-
Palavras-chave: dc.subjectIdentification-
Palavras-chave: dc.subjectLactococcosis-
Palavras-chave: dc.subjectSerotyping-
Título: dc.titleGenetic characterization of lactococcosis-causing bacteria isolated from Brazilian native fish species-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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