Assessment of Oxidative Stress Biomarkers in Rat Blood

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.creatorSinzato, Yuri K.-
Autor(es): dc.creatorRodrigues, Thiago-
Autor(es): dc.creatorCruz, Larissa L.-
Autor(es): dc.creatorBarco, Vinicius S.-
Autor(es): dc.creatorSouza, Maysa R.-
Autor(es): dc.creatorVolpato, Gustavo T.-
Autor(es): dc.creatorDamasceno, Debora C.-
Data de aceite: dc.date.accessioned2025-08-21T21:59:55Z-
Data de disponibilização: dc.date.available2025-08-21T21:59:55Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-03-05-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.21769/BioProtoc.4626-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300186-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300186-
Descrição: dc.descriptionRedox status assessments are time-consuming, require a large volume of samples and great reagent amounts, and are not adequately described for methodological reproducibility. Here, the objective was to standardize redox balance determination, based on previously described spectrophotometric tests in pregnant rats, to improve precision, time dispensed, and the volume of samples and reagents, while maintaining accuracy and adequate cost benefits. This protocol summarizes oxidative stress markers, which focus on spectrophotometric tests for the assessment of thiobarbituric acid-reactive substances, reduced thiol groups, and hydrogen peroxide, as well as the antioxidant activity of superoxide dismutase, glutathione peroxidase, and catalase in washed erythrocyte and serum samples from full-term pregnant rats. For non-pregnant rats and other species, it is necessary to standardize these determinations, especially the sample volume. All measurements were normalized by the estimated protein concentrations in each sample. To establish optimum conditions for the reproducibility of the proposed methods, we describe all changes made in each assay's steps based on the reference method reassessed for the new standardizations. Furthermore, the calculations of the concentrations or activities of each marker are presented. Thus, we demonstrate that the analysis of serum samples is easier and faster, but it is impossible to detect catalase activity. Furthermore, the proposed methods can be applied for redox balance determination, especially using smaller reagent amounts and lower sample volumes in lesser time without losing accuracy, as is required in obtaining samples during rat pregnancy.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionSao Paulo State Univ Unesp, Botucatu Med Sch, Lab Expt Res Gynecol & Obstet, Botucatu, SP, Brazil-
Descrição: dc.descriptionFed Univ ABC UFABC, Ctr Nat & Human Sci CCNH, Santo Andre, SP, Brazil-
Descrição: dc.descriptionFed Univ Mato Grosso UFMT, Inst Biol & Hlth Sci, Lab Syst Physiol & Reprod Toxicol, Barra Garcas, MT, Brazil-
Descrição: dc.descriptionSao Paulo State Univ Unesp, Botucatu Med Sch, Lab Expt Res Gynecol & Obstet, Botucatu, SP, Brazil-
Descrição: dc.descriptionFAPESP: 2016/25207-5-
Descrição: dc.descriptionCAPES: 001-
Formato: dc.format26-
Idioma: dc.languageen-
Publicador: dc.publisherBio-protocol-
Relação: dc.relationBio-protocol-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectOxidative stress-
Palavras-chave: dc.subjectLipid peroxidation-
Palavras-chave: dc.subjectBiomarker-
Palavras-chave: dc.subjectRedox status-
Palavras-chave: dc.subjectPregnancy-
Título: dc.titleAssessment of Oxidative Stress Biomarkers in Rat Blood-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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