Development and comparative assessment of RT-qPCR and duplex RT-LAMP assays for the monitoring of Aichi virus A (AiV-A) in untreated wastewater samples

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorEcosciences Precinct-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorXiamen University-
Autor(es): dc.contributorLouisiana State University-
Autor(es): dc.creatordo Nascimento, Mariah C.A.-
Autor(es): dc.creatorSmith, Wendy J.M.-
Autor(es): dc.creatorLiu, Yawen-
Autor(es): dc.creatorSimpson, Stuart L.-
Autor(es): dc.creatorBivins, Aaron-
Autor(es): dc.creatorRahal, Paula-
Autor(es): dc.creatorAhmed, Warish-
Data de aceite: dc.date.accessioned2025-08-21T22:08:11Z-
Data de disponibilização: dc.date.available2025-08-21T22:08:11Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-19-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.scitotenv.2024.175440-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300176-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300176-
Descrição: dc.descriptionDiverse enteric pathogens, transmitted through human and animal feces, can cause gastroenteritis. Enteric viruses, such as human Aichi virus, specifically genotype A (AiV-A), are emerging pathogens that cause illnesses even at low doses and are spreading globally. This research developed a reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting the 3CD junction and a reverse transcription colorimetric loop-mediated isothermal amplification (RT-cLAMP) duplex assay targeting junctions 2BC and 3CD of the AiV-A genome for rapid and sensitive detection of this virus in metropolitan and regional wastewater samples in Queensland, Australia. The performance of these assays was evaluated using control materials and by analyzing wastewater samples. In serially diluted control materials, RT-qPCR provided quantifiable data (mean 1.51 log10 GC/2 μL of nucleic acid) down to a dilution of 1 × 10−5 pg/μL. In comparison, the duplex RT-cLAMP assay detected down to 1 × 10−4 pg/μL, indicating that its sensitivity was one order of magnitude less than that of RT-qPCR. Of the 38 wastewater samples from 38 metropolitan and regional wastewater treatment plants (WWTPs) in Queensland, Australia, 21 (55.3 %) tested positive by RT-qPCR with concentrations ranging from 3.60 to 6.23 log10 GC/L. In contrast, only 15 (39.5 %) of 38 wastewater samples were positive using the duplex RT-cLAMP assay. The methods demonstrated substantial qualitative agreement (κ = 0.730), with a concordance of 86.5 %, demonstrating the reliability of RT-cLAMP for detecting AiV-A in wastewater samples. The duplex RT-cLAMP assay, despite demonstrating reduced detection sensitivity, has proven effective and holds promise as a supplementary approach, especially in settings with limited resources where rapid and affordable testing is crucial.-
Descrição: dc.descriptionCSIRO Environment Ecosciences Precinct, 41 Boggo Road-
Descrição: dc.descriptionDepartment of Biology São Paulo State University — UNESP, São José do Rio Preto-
Descrição: dc.descriptionState Key Laboratory of Marine Environmental Science College of the Environment & Ecology Xiamen University-
Descrição: dc.descriptionDepartment of Civil & Environmental Engineering Louisiana State University-
Descrição: dc.descriptionDepartment of Biology São Paulo State University — UNESP, São José do Rio Preto-
Idioma: dc.languageen-
Relação: dc.relationScience of the Total Environment-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAichi virus-
Palavras-chave: dc.subjectEnteric virus-
Palavras-chave: dc.subjectLAMP-
Palavras-chave: dc.subjectRT-qPCR-
Palavras-chave: dc.subjectWastewater surveillance-
Título: dc.titleDevelopment and comparative assessment of RT-qPCR and duplex RT-LAMP assays for the monitoring of Aichi virus A (AiV-A) in untreated wastewater samples-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.