De novo assembly and characterization of a highly degenerated ZW sex chromosome in the fish Megaleporinus macrocephalus

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorWellcome Sanger Institute-
Autor(es): dc.contributorUniversity of Edinburgh-
Autor(es): dc.contributorSão Paulo Agency of Agribusiness and Technology (APTA)-
Autor(es): dc.contributorLPGP-
Autor(es): dc.creatorSouza-Borges, Carolina Heloisa-
Autor(es): dc.creatorUtsunomia, Ricardo-
Autor(es): dc.creatorVarani, Alessandro M.-
Autor(es): dc.creatorUliano-Silva, Marcela-
Autor(es): dc.creatorLira, Lieschen Valeria G.-
Autor(es): dc.creatorButzge, Arno J.-
Autor(es): dc.creatorGomez Agudelo, John F.-
Autor(es): dc.creatorManso, Shisley-
Autor(es): dc.creatorFreitas, Milena V.-
Autor(es): dc.creatorAriede, Raquel B.-
Autor(es): dc.creatorMastrochirico-Filho, Vito A.-
Autor(es): dc.creatorPenaloza, Carolina-
Autor(es): dc.creatorBarria, Agustín-
Autor(es): dc.creatorPorto-Foresti, Fábio-
Autor(es): dc.creatorForesti, Fausto-
Autor(es): dc.creatorHattori, Ricardo-
Autor(es): dc.creatorGuiguen, Yann-
Autor(es): dc.creatorHouston, Ross D.-
Autor(es): dc.creatorHashimoto, Diogo Teruo-
Data de aceite: dc.date.accessioned2025-08-21T15:16:08Z-
Data de disponibilização: dc.date.available2025-08-21T15:16:08Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/gigascience/giae085-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300120-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300120-
Descrição: dc.descriptionBackground.Megaleporinus macrocephalus (piauçu) is a Neotropical fish within Characoidei that presents a well-established heteromorphic ZZ/ZW sex determination system and thus constitutes a good model for studying W and Z chromosomes in fishes. We used PacBio reads and Hi-C to assemble a chromosome-level reference genome for M. macrocephalus. We generated family segregation information to construct a genetic map, pool sequencing of males and females to characterize its sex system, and RNA sequencing to highlight candidate genes of M. macrocephalus sex determination. Results. The reference genome of M. macrocephalus is 1,282,030,339 bp in length and has a contig and scaffold N50 of 5.0 Mb and 45.03 Mb, respectively. In the sex chromosome, based on patterns of recombination suppression, coverage, FST, and sex-specific SNPs, we distinguished a putative W-specific region that is highly differentiated, a region where Z and W still share some similarities and is undergoing degeneration, and the PAR. The sex chromosome gene repertoire includes genes from the TGF-β family (amhr2, bmp7) and the Wnt/β-catenin pathway (wnt4, wnt7a), some of which are differentially expressed. Conclusions. The chromosome-level genome of piauçu exhibits high quality, establishing a valuable resource for advancing research within the group. Our discoveries offer insights into the evolutionary dynamics of Z and W sex chromosomes in fish, emphasizing ongoing degenerative processes and indicating complex interactions between Z and W sequences in specific genomic regions. Notably, amhr2 and bmp7 are potential candidate genes for sex determination in M. macrocephalus.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionAquaculture Center of Unesp São Paulo State University (Unesp), SP-
Descrição: dc.descriptionSchool of Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionSchool of Agricultural and Veterinary Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionWellcome Sanger Institute-
Descrição: dc.descriptionThe Roslin Institute University of Edinburgh, Easter Bush-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionSão Paulo Agency of Agribusiness and Technology (APTA), SP-
Descrição: dc.descriptionINRAE LPGP-
Descrição: dc.descriptionAquaculture Center of Unesp São Paulo State University (Unesp), SP-
Descrição: dc.descriptionSchool of Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionSchool of Agricultural and Veterinary Sciences São Paulo State University (Unesp), SP-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (Unesp), SP-
Idioma: dc.languageen-
Relação: dc.relationGigaScience-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectamhr2-
Palavras-chave: dc.subjectchromosome-level genome-
Palavras-chave: dc.subjectsex chromosome assembly-
Palavras-chave: dc.subjectsex determination-
Título: dc.titleDe novo assembly and characterization of a highly degenerated ZW sex chromosome in the fish Megaleporinus macrocephalus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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