Impedimetric Immunosensor for 5-Methylcytosine Detection Based on a Poly(o-phenylenediamine)-Encapsulated Gold Nanoparticle Platform

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPizzol, Pedro C. M.-
Autor(es): dc.creatorPortugal, Miquéias L.-
Autor(es): dc.creatorTrevizan, Heitor F.-
Autor(es): dc.creatorSeraphim, Patrícia Monteiro-
Autor(es): dc.creatorTeixeira, Marcos F. S.-
Data de aceite: dc.date.accessioned2025-08-21T22:36:38Z-
Data de disponibilização: dc.date.available2025-08-21T22:36:38Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1021/acsaelm.5c00216-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/300071-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/300071-
Descrição: dc.descriptionA label-free impedimetric immunosensor for the detection of 5-methylcytosine (5-mC), a key epigenetic marker, was developed based on a one-step electropolymerized nanocomposite of poly(o-phenylenediamine) (poly(o-PD)) and gold nanoparticles (AuNPs). The nanocomposite film was electropolymerized onto a screen-printed electrode (SPE) with a gold working electrode area of 0.0078 cm2. The system was characterized by cyclic voltammetry and electrochemical impedance spectroscopy (EIS). The EIS measurements, including Nyquist, Bode, and complex capacitance plots, confirmed the successful formation of the nanocomposite and its stepwise modification with glutaraldehyde, anti-5-methylcytosine antibody (Ab-5mC), and bovine serum albumin (BSA). The immunosensor utilized the inherent redox activity of the poly(o-PD) toward dissolved oxygen as a transduction mechanism, eliminating the need for external redox mediators. The binding of 5-mC to the immobilized Ab-5mC hindered the access of dissolved oxygen to the redox-active phenazine-like units within the poly(o-PD) matrix, resulting in a measurable increase in the charge transfer resistance. The immunosensor exhibited a linear response to the logarithm of 5-mC concentration in the range of 2.5 to 160 pg mL-1, with a low limit of detection (LOD) of 1.73 and 1.18 pg mL-1 when using the imaginary and absolute impedance, respectively. The incorporation of AuNPs significantly enhanced the electrochemically active surface area and improved the electron transfer kinetics, contributing to the high sensitivity of the immunosensor. This work demonstrates the potential of a one-step electropolymerized poly(o-PD)-AuNP nanocomposite for the development of simple, label-free, and sensitive impedimetric immunosensors for epigenetic biomarker detection.-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, SP,-
Descrição: dc.descriptionDepartment of Physiotherapy School of Science and Technology Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry School of Science and Technology Sao Paulo State University (UNESP), Rua Roberto Simonsen, 305, SP,-
Descrição: dc.descriptionDepartment of Physiotherapy School of Science and Technology Sao Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationACS Applied Electronic Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectdissolved oxygen-
Palavras-chave: dc.subjectgold nanocomposite-
Palavras-chave: dc.subjectimpedimetric sensor-
Palavras-chave: dc.subjectmethylated DNA-
Palavras-chave: dc.subjectone-step electropolymerization-
Título: dc.titleImpedimetric Immunosensor for 5-Methylcytosine Detection Based on a Poly(o-phenylenediamine)-Encapsulated Gold Nanoparticle Platform-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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