Mining microbial tapestry using high-throughput sequencing and In silico analysis of Trehalose synthase (TreS) derived from hot spring metagenome

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Autor(es): dc.contributorLovely Professional University-
Autor(es): dc.contributorUniversity of Jammu-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorBrazil University-
Autor(es): dc.creatorWani, Atif Khurshid-
Autor(es): dc.creatorChopra, Chirag-
Autor(es): dc.creatorSingh, Reena-
Autor(es): dc.creatorAhmad, Suhail-
Autor(es): dc.creatorAmérico-Pinheiro, Juliana Heloisa Pinê-
Data de aceite: dc.date.accessioned2025-08-21T17:11:46Z-
Data de disponibilização: dc.date.available2025-08-21T17:11:46Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bcab.2023.102829-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299989-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299989-
Descrição: dc.descriptionUnderstanding the microbial diversity and research trends using metagenomics is important for biotechnological and environmental applications. In this study, we carried out bibliometric analysis on hot metagenomics to study the research trends. The high-throughput metagenome sequencing was used to investigate the taxonomic profile of Rajouri hot spring (RHS) located at 33.1829° N, 75.1441° with 3445 feet above the sea level. The isolated metagenomic DNA (mDNA) was subjected to sequencing using Illumina technology, and the data was processed using QIIME for taxonomic classification and diversity analysis. The results showed a high level of diversity within the microbial communities with Proteobacteria (41.03%), Actinobacteria (16.7%), and Firmicutes (7.42%) as the dominant phyla. The Shannon (3.28), Simpson (0.053), and Chao1 (198.5) indices indicate the microbial richness in RHS suggesting a more stable and resilient ecosystem. The Trehalose synthase (TreS) gene, responsible for catalyzing the conversion of maltose to trehalose, was subjected to PCR amplification, DNA sequencing, and subsequent computational analysis. Through these investigations, it was determined that the TreS protein is composed of 169 amino acids, displaying a globular conformation and possessing hydrophilic characteristics. The advancements in high-throughput sequencing technology will enable more comprehensive and accurate analysis of the hot spring metagenome, leading to a deeper understanding of the microbial communities and their interactions.-
Descrição: dc.descriptionSchool of Bioengineering and Biosciences Lovely Professional University, Punjab-
Descrição: dc.descriptionDepartment of Remote Sensing and GIS University of Jammu-
Descrição: dc.descriptionDepartment of Forest Science Soils and Environment School of Agronomic Sciences São Paulo State University (UNESP), Ave. Universitária, 3780, SP-
Descrição: dc.descriptionGraduate Program in Environmental Sciences Brazil University, Street Carolina Fonseca, 584, SP-
Descrição: dc.descriptionDepartment of Forest Science Soils and Environment School of Agronomic Sciences São Paulo State University (UNESP), Ave. Universitária, 3780, SP-
Idioma: dc.languageen-
Relação: dc.relationBiocatalysis and Agricultural Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHot spring-
Palavras-chave: dc.subjectHydrolase-
Palavras-chave: dc.subjectMetagenomics-
Palavras-chave: dc.subjectMicrobial diversity-
Palavras-chave: dc.subjectSequencing-
Palavras-chave: dc.subjectTrehalose synthase-
Título: dc.titleMining microbial tapestry using high-throughput sequencing and In silico analysis of Trehalose synthase (TreS) derived from hot spring metagenome-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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