Expression of MHC I Isoforms in Bovine Placentomes: Impact of Cloning

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidad de Concepción-
Autor(es): dc.contributorUniversity of Marília (UNIMAR)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Georgia-
Autor(es): dc.creatorBarreto, Rodrigo da Silva Nunes-
Autor(es): dc.creatorMançanares, Ana Carolina Furlanetto-
Autor(es): dc.creatorMiglino, Maria Angelica-
Autor(es): dc.creatorMeirelles, Flávio Vieira-
Autor(es): dc.creatorOliveira, Lilian de Jesus-
Data de aceite: dc.date.accessioned2025-08-21T21:47:17Z-
Data de disponibilização: dc.date.available2025-08-21T21:47:17Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/vetsci12030196-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299936-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299936-
Descrição: dc.descriptionMajor histocompatibility complex class I (MHC-I) gene expression in the placenta is modulated to tailor the maternal immune response to fetal antigens during pregnancy. This study evaluated MHC-I expression through immunohistochemistry (IHC) using an anti-mouse preimplantation embryo development (PED) clone Qa-2 and anti-bovine leukocyte antigen I (BoLA) monoclonal antibody clone IL-A88 (n = 23), as well as RT-qPCR (n = 17) for classical and non-classical (BoLA-NC) genes in control and cloned bovine placentomes during early and near-term gestation. Control samples showed minimal Qa-2 protein expression in early gestation, with intense labeling in trophoblasts and the maternal uterine epithelium near term. In contrast, cloned samples exhibited intense Qa-2 labeling in both maternal and trophoblastic epithelia at both stages, while trophoblast giant cells (TGCs), located apposed to the maternal epithelium, showed no labeling. Control samples exhibited intense IL-A88 labeling in the maternal epithelium at both stages. In cloned samples, weak to no labeling was observed in early gestation, with intense labeling in maternal and fetal epithelium near term. RT-qPCR revealed significant upregulation of BoLA-NC3 in early gestation, with sustained elevated expression in cloned samples in the near term. These findings suggest that altered BoLA protein expression and gene regulation in cloned pregnancies may contribute to pregnancy complications and increased losses.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionRB Lab College of Agricultural and Veterinary Sciences Sao Paulo State University, SP-
Descrição: dc.descriptionDepartment of Animal Science Faculty of Veterinary Sciences Universidad de Concepción-
Descrição: dc.descriptionDepartment of Veterinary Medicine University of Marília (UNIMAR), SP-
Descrição: dc.descriptionDepartment of Veterinary Medicine Faculty of Animal Sciences and Food Engineering University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Pathology College of Veterinary Medicine University of Georgia-
Descrição: dc.descriptionRB Lab College of Agricultural and Veterinary Sciences Sao Paulo State University, SP-
Descrição: dc.descriptionCAPES: 2009/06702-1-
Descrição: dc.descriptionFAPESP: 2021/05445-7-
Descrição: dc.descriptionFAPESP: CEPID-CTC 2013/08135-2-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBoLA-
Palavras-chave: dc.subjectbovine placenta-
Palavras-chave: dc.subjectchorionic villi-
Palavras-chave: dc.subjectcloned bovine-
Palavras-chave: dc.subjectIL-A88-
Palavras-chave: dc.subjectQa-2-
Título: dc.titleExpression of MHC I Isoforms in Bovine Placentomes: Impact of Cloning-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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