Nanoemulsion Increases the Antifungal Activity of Amphotericin B against Four Candida auris Clades: In Vitro and In Vivo Assays

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Autor(es): dc.contributorHealth Research Institute La Fe-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity and Polytechnic La Fe Hospital-
Autor(es): dc.contributorUniversity of Valencia-
Autor(es): dc.contributorLa Fe Hospital-
Autor(es): dc.creatorMarena, Gabriel Davi-
Autor(es): dc.creatorRuiz-Gaitán, Alba-
Autor(es): dc.creatorGarcia-Bustos, Victor-
Autor(es): dc.creatorTormo-Mas, María Ángeles-
Autor(es): dc.creatorPérez-Royo, Jose Manuel-
Autor(es): dc.creatorLópez, Alejandro-
Autor(es): dc.creatorBernarbe, Patricia-
Autor(es): dc.creatorPérez Ruiz, María Dolores-
Autor(es): dc.creatorZaragoza Macian, Lara-
Autor(es): dc.creatorVicente Saez, Carmen-
Autor(es): dc.creatorAvalos Mansilla, Antonia-
Autor(es): dc.creatorGómez, Eulogio Valentín-
Autor(es): dc.creatorCarvalho, Gabriela Corrêa-
Autor(es): dc.creatorBauab, Tais Maria-
Autor(es): dc.creatorChorilli, Marlus-
Autor(es): dc.creatorPemán, Javier-
Data de aceite: dc.date.accessioned2025-08-21T20:57:50Z-
Data de disponibilização: dc.date.available2025-08-21T20:57:50Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/microorganisms11071626-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299906-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299906-
Descrição: dc.descriptionCandida auris is an emerging yeast of worldwide interest due to its antifungal resistance and mortality rates. The aim of this study was to analyse the in vitro and in vivo antifungal activity of a nanoemulsion loaded with amphotericin B (NEA) against planktonic cells and biofilm of C. auris clinical isolates belonging to four different clades. In vivo assays were performed using the Galleria mellonella model to analyse antifungal activity and histopathological changes. The in vitro results showed that NEA exhibited better antifungal activity than free amphotericin B (AmB) in both planktonic and sessile cells, with >31% inhibition of mature biofilm. In the in vivo assays, NEA demonstrated superior antifungal activity in both haemolymph and tissue. NEA reduced the fungal load in the haemolymph more rapidly and with more activity in the first 24 h after infection. The histological analysis of infected larvae revealed clusters of yeast, immune cells, melanisation, and granulomas. In conclusion, NEA significantly improved the in vitro and in vivo antifungal activity of AmB and could be considered a promising therapy for C. auris infections.-
Descrição: dc.descriptionSevere Infection Research Group Health Research Institute La Fe-
Descrição: dc.descriptionDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Medical Microbiology University and Polytechnic La Fe Hospital-
Descrição: dc.descriptionDepartment of Pathology Faculty of Medicine and Dentistry University of Valencia-
Descrição: dc.descriptionDepartment of Pathological Anatomy La Fe Hospital-
Descrição: dc.descriptionDepartment of Microbiology and Ecology University of Valencia-
Descrição: dc.descriptionDepartment of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMicroorganisms-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectamphotericin B-
Palavras-chave: dc.subjectCandida auris-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjectinfectious disease-
Palavras-chave: dc.subjectnanoemulsion-
Título: dc.titleNanoemulsion Increases the Antifungal Activity of Amphotericin B against Four Candida auris Clades: In Vitro and In Vivo Assays-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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