From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Marília (UNIMAR)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorAgricultural Sciences—Federal University of Vale do São Francisco (UNIVASF)-
Autor(es): dc.contributorUniversity Center of Adamantina (FAI)-
Autor(es): dc.contributorUniversity of Marilia (UNIMAR)-
Autor(es): dc.creatordos Santos, Ana Caroline-
Autor(es): dc.creatorde Andrade, Livia Maria Barbosa-
Autor(es): dc.creatorCandelária, Raí André Querino-
Autor(es): dc.creatorde Carvalho, Juliana Casanovas-
Autor(es): dc.creatorValbão, Maria Carolina Miglino-
Autor(es): dc.creatorBarreto, Rodrigo da Silva Nunes-
Autor(es): dc.creatorde Faria, Marcelo Domingues-
Autor(es): dc.creatorBuchaim, Rogerio Leone-
Autor(es): dc.creatorBuchaim, Daniela Vieira-
Autor(es): dc.creatorMiglino, Maria Angelica-
Data de aceite: dc.date.accessioned2025-08-21T17:19:00Z-
Data de disponibilização: dc.date.available2025-08-21T17:19:00Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/bioengineering12010052-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299775-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299775-
Descrição: dc.descriptionThe shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool for producing ideal scaffolds for the recellularization and implantation of new tissue in damaged areas. In order to be classified as an ideal scaffold, it must be acellular, preserving its proteins and physical characteristics necessary for cell adhesion. This study aimed to develop a decellularization protocol for pig ear cartilage and evaluate the integrity of the ECM. Four tests were performed using different methods and protocols, with four pig ears from which the skin and subcutaneous tissue were removed, leaving only the cartilage. The most efficient protocol was the combination of trypsin with a sodium hydroxide solution (0.2 N) and SDS (1%) without altering the ECM conformation or the collagen architecture. In conclusion, it was observed that auricular cartilage is difficult to decellularize, influenced by material size, exposure time, and the composition of the solution. Freezing and thawing did not affect the procedure. The sample thickness significantly impacted the decellularization time.-
Descrição: dc.descriptionGraduate Program in Anatomy of Domestic and Wild Animals Faculty of Veterinary Medicine and Animal Science University of São Paulo (FMVZ/USP)-
Descrição: dc.descriptionMedical School University of Marília (UNIMAR)-
Descrição: dc.descriptionDepartment of Animal Morphology and Physiology Faculty of Agricultural and Veterinary Sciences São Paulo State University-
Descrição: dc.descriptionDepartment of Animal Anatomy Agricultural Sciences—Federal University of Vale do São Francisco (UNIVASF)-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry (FOB/USP) University of São Paulo-
Descrição: dc.descriptionMedical School University Center of Adamantina (FAI)-
Descrição: dc.descriptionPostgraduate Program in Structural and Functional Interactions in Rehabilitation Postgraduate Department University of Marilia (UNIMAR)-
Descrição: dc.descriptionPostgraduate Program in Animal Health Production and Environment University of Marilia (UNIMAR)-
Descrição: dc.descriptionDepartment of Animal Morphology and Physiology Faculty of Agricultural and Veterinary Sciences São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationBioengineering-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiological scaffolds-
Palavras-chave: dc.subjectcartilaginous tissue-
Palavras-chave: dc.subjectcollagen-
Palavras-chave: dc.subjectenzymatic decellularization-
Palavras-chave: dc.subjectextracellular matrix-
Palavras-chave: dc.subjectrecellularization-
Palavras-chave: dc.subjecttissue engineering-
Título: dc.titleFrom Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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