Effects of natural antimicrobial compounds propolis and copaiba on periodontal ligament fibroblasts, molecular docking, and in vivo study in Galleria mellonella

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Groningen-
Autor(es): dc.contributorFederal University of ABC-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorPedrinha, Victor Feliz-
Autor(es): dc.creatorSantos, Letícia Martins-
Autor(es): dc.creatorGonçalves, Carolina Passarelli-
Autor(es): dc.creatorGarcia, Maíra Terra-
Autor(es): dc.creatorLameira, Osmar Alves-
Autor(es): dc.creatorQueiroga, Carmen Lucia-
Autor(es): dc.creatorMarcucci, Maria Cristina-
Autor(es): dc.creatorShahbazi, Mohammad-Ali-
Autor(es): dc.creatorSharma, Prashant Kumar-
Autor(es): dc.creatorJunqueira, Juliana Campos-
Autor(es): dc.creatorSipert, Carla Renata-
Autor(es): dc.creatorde Andrade, Flaviana Bombarda-
Data de aceite: dc.date.accessioned2025-08-21T18:57:46Z-
Data de disponibilização: dc.date.available2025-08-21T18:57:46Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2024.116139-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299748-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299748-
Descrição: dc.descriptionRoot canal treatment addresses infectious processes that require control. Occasionally, the radicular pulp is vital and inflamed, presenting a superficial infection. To preserve pulpal remnants, conservative procedures have gained favor, employing anti-inflammatory medications. This study investigated the effects of propolis (PRO), and copaiba oil-resin (COR) associated with hydrocortisone (H) and compared their impact to that of Otosporin® concerning cytotoxic and genotoxic activity, cytokine detection, and toxicity in the Galleria mellonella model. Human periodontal ligament fibroblasts (PDLFs) were exposed to drug concentrations and evaluated by the MTT assay. Associations were tested from concentrations that did not compromise cell density. Genotoxicity was evaluated through micronucleus counting, while cytokines IL-6 and TGF-β1 were detected in the cell supernatant using ELISA. Molecular docking simulations were conducted, considering the major compounds identified in PRO, COR, and H. Increasing concentrations of PRO and COR were assessed for acute toxicity in Galleria mellonella model. Cellular assays were analyzed using one-way ANOVA followed by Tukey tests, while larval survivals were evaluated using the Log-rank (Mantel-Cox) test (α = 0.05). PRO and COR promoted PDLFs proliferation, even in conjunction with H. No changes in cell metabolism were observed concerning cytokine levels. The tested materials induce the release of AT1R, proliferating the PDFLs through interactions. PRO and COR had low toxicity in larvae, suggesting safety at tested levels. These findings endorse the potential of PRO and COR in endodontics and present promising applications across medical domains, such as preventive strategies in inflammation, shedding light on their potential development into commercially available drugs.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo (FOB – USP), Bauru-
Descrição: dc.descriptionDepartment of Biomaterials and Biomedical Technology (BBT) University Medical Center Groningen (UMCG) University of Groningen-
Descrição: dc.descriptionDepartment of Operative Dentistry School of Dentistry University of São Paulo (FO-USP)-
Descrição: dc.descriptionCenter for Natural and Human Sciences Federal University of ABC, SP-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São José dos Campos-
Descrição: dc.descriptionLaboratory of Biotechnology EMBRAPA Eastern Amazon, Belém-
Descrição: dc.descriptionState University of Campinas CPQBA Division of Phytochemistry, Campinas-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São José dos Campos-
Descrição: dc.descriptionFAPESP: 2010/20186–3-
Descrição: dc.descriptionFAPESP: 2020/12726–0-
Idioma: dc.languageen-
Relação: dc.relationBiomedicine and Pharmacotherapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial agents-
Palavras-chave: dc.subjectDental pulp diseases-
Palavras-chave: dc.subjectMolecular Docking-
Palavras-chave: dc.subjectPropolis-
Palavras-chave: dc.subjectToxicity-
Título: dc.titleEffects of natural antimicrobial compounds propolis and copaiba on periodontal ligament fibroblasts, molecular docking, and in vivo study in Galleria mellonella-
Tipo de arquivo: dc.typelivro digital-
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