Exosomes modified with anti-MEK1 siRNA lead to an effective silencing of triple negative breast cancer cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Minho-
Autor(es): dc.contributorLABBELS—Associate Laboratory-
Autor(es): dc.contributorICVS/3B's–PT Government Associate Laboratory-
Autor(es): dc.contributorUniversity of Texas MD Anderson Cancer Center-
Autor(es): dc.contributorBarretos Cancer Hospital-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade do Porto-
Autor(es): dc.contributorUniversity of Coimbra-
Autor(es): dc.contributorFaculty of Medicine (Polo 1)-
Autor(es): dc.contributorPólo das Ciências da Saúde-
Autor(es): dc.contributorRice University-
Autor(es): dc.contributorBaylor College of Medicine-
Autor(es): dc.creatorFerreira, Débora-
Autor(es): dc.creatorSantos-Pereira, Cátia-
Autor(es): dc.creatorCosta, Marta-
Autor(es): dc.creatorAfonso, Julieta-
Autor(es): dc.creatorYang, Sujuan-
Autor(es): dc.creatorHensel, Janine-
Autor(es): dc.creatorMcAndrews, Kathleen M.-
Autor(es): dc.creatorLongatto-Filho, Adhemar-
Autor(es): dc.creatorFernandes, Rui-
Autor(es): dc.creatorMelo, Joana B.-
Autor(es): dc.creatorBaltazar, Fátima-
Autor(es): dc.creatorMoreira, João N.-
Autor(es): dc.creatorKalluri, Raghu-
Autor(es): dc.creatorRodrigues, Ligia R.-
Data de aceite: dc.date.accessioned2025-08-21T21:45:04Z-
Data de disponibilização: dc.date.available2025-08-21T21:45:04Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bioadv.2023.213643-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299722-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299722-
Descrição: dc.descriptionTriple negative breast cancer (TNBC) is a highly heterogenous disease not sensitive to endocrine or HER2 therapy and standardized treatment regimens are still missing. Therefore, development of novel TNBC treatment approaches is of utmost relevance. Herein, the potential of MAPK/ERK downregulation by RNAi-based therapeutics in a panel of mesenchymal stem-like TNBC cell lines was uncovered. Our data revealed that suppression of one of the central nodes of this signaling pathway, MEK1, affects proliferation, migration, and invasion of TNBC cells, that may be explained by the reversion of the epithelial-mesenchymal transition phenotype, which is facilitated by the MMP-2/MMP-9 downregulation. Moreover, an exosome-based system was successfully generated for the siRNA loading (iExoMEK1). Our data suggested absence of modification of the physical properties and general integrity of the iExoMEK1 comparatively to the unmodified counterparts. Such exosome-mediated downregulation of MEK1 led to a tumor regression accompanied by a decrease of angiogenesis using the chick chorioallantoic-membrane model. Our results highlight the potential of the targeting of MAPK/ERK cascade as a promising therapeutic approach against TNBC.-
Descrição: dc.descriptionEuropean Regional Development Fund-
Descrição: dc.descriptionEuropean Social Fund-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia-
Descrição: dc.descriptionCEB-Centre of Biological Engineering University of Minho, Campus de Gualtar-
Descrição: dc.descriptionLABBELS—Associate Laboratory-
Descrição: dc.descriptionLife and Health Sciences Research Institute (ICVS) University of Minho, Campus of Gualtar-
Descrição: dc.descriptionICVS/3B's–PT Government Associate Laboratory-
Descrição: dc.descriptionDepartment of Cancer Biology Metastasis Research Center University of Texas MD Anderson Cancer Center-
Descrição: dc.descriptionMolecular Oncology Research Center Barretos Cancer Hospital, São Paulo-
Descrição: dc.descriptionLaboratory of Medical Investigation (LIM 14) Faculty of Medicine São Paulo State University-
Descrição: dc.descriptionHEMS—Histology and Electron Microscopy Service IBMC/I3S Universidade do Porto-
Descrição: dc.descriptionCytogenetics and Genomics Laboratory Faculty of Medicine University of Coimbra-
Descrição: dc.descriptionCenter of Investigation on Environment Genetics and Oncobiology Faculty of Medicine University of Coimbra-
Descrição: dc.descriptionCNC–Center for Neurosciences and Cell Biology Center for Innovative Biomedicine and Biotechnology (CIBB) University of Coimbra Faculty of Medicine (Polo 1), Rua Larga-
Descrição: dc.descriptionUniv Coimbra–University of Coimbra CIBB Faculty of Pharmacy Pólo das Ciências da Saúde, Azinhaga de Santa Comba-
Descrição: dc.descriptionSchool of Bioengineering Rice University-
Descrição: dc.descriptionDepartment of Molecular and Cellular Biology Baylor College of Medicine-
Descrição: dc.descriptionLaboratory of Medical Investigation (LIM 14) Faculty of Medicine São Paulo State University-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: PD/BD/128032/2016-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: SFRH/BPD/116784/2016-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: UIDB/50026/2020-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: UIDP/50026/2020-
Idioma: dc.languageen-
Relação: dc.relationBiomaterials Advances-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectExosome-mediated silencing-
Palavras-chave: dc.subjectMAPK/ERK cascade-
Palavras-chave: dc.subjectMEK1-
Palavras-chave: dc.subjectsiRNA-
Palavras-chave: dc.subjectTNBC-
Título: dc.titleExosomes modified with anti-MEK1 siRNA lead to an effective silencing of triple negative breast cancer cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.