EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorCenter for Interdisciplinary Research in Health (CIIS)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFaculty of Dentistry-
Autor(es): dc.creatorDuque, Cristiane-
Autor(es): dc.creatorRabelo, Rafaela Laruzo-
Autor(es): dc.creatorBraga, Gabriela Pacheco de Almeida-
Autor(es): dc.creatorMendes Soares, Igor Paulino-
Autor(es): dc.creatorde Souza, Maria Eduarda-
Autor(es): dc.creatorChrisostomo, Daniela Alvim-
Autor(es): dc.creatorHebling, Josimeri-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorPrakki, Anuradha-
Autor(es): dc.creatorRegasini, Luís Octavio-
Data de aceite: dc.date.accessioned2025-08-21T17:02:47Z-
Data de disponibilização: dc.date.available2025-08-21T17:02:47Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1155/sci5/3233536-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299665-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299665-
Descrição: dc.descriptionOdontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin–pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of Alpl, Mmp2, Mmp9, Dmp1, and Dspp genes in odontoblast-like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25 μM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of Dmp1 and Dspp highly increased with taxifolin treatment, and Alpl expression was increased for both taxifolin and EGCG groups, without difference between them. Mmp2 and Mmp9 expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early- and late-stage dentinogenesis genes.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia-
Descrição: dc.descriptionUniversidade Católica Portuguesa (UCP) Faculty of Dental Medicine Center for Interdisciplinary Research in Health (CIIS), Estrada da Circunvalação, s/n, PRT-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Preventive and Restorative Dentistry Araçatuba Dental School R. José Bonifácio 1193, unesp.br, BRA-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Dental Materials and Prosthodontics Araraquara School of Dentistry, R. Humaitá, 1680-
Descrição: dc.descriptionUniversity of Toronto Department of Clinical Sciences-Restorative Dentistry Faculty of Dentistry, 124 Edward St-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Morphology Genetics Orthodontics and Pediatric Dentistry Araraquara School of Dentistry, R. Humaitá, 1680-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Physiology and Pathology Araraquara School of Dentistry, R. Humaitá, 1680-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences, R. Cristóvão Colombo 2265-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Preventive and Restorative Dentistry Araçatuba Dental School R. José Bonifácio 1193, unesp.br, BRA-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Dental Materials and Prosthodontics Araraquara School of Dentistry, R. Humaitá, 1680-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Morphology Genetics Orthodontics and Pediatric Dentistry Araraquara School of Dentistry, R. Humaitá, 1680-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Physiology and Pathology Araraquara School of Dentistry, R. Humaitá, 1680-
Descrição: dc.descriptionSão Paulo State University (UNESP) Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences, R. Cristóvão Colombo 2265-
Descrição: dc.descriptionFAPESP: 2017/10940-1-
Descrição: dc.descriptionNatural Sciences and Engineering Research Council of Canada: 2018-06489-
Descrição: dc.descriptionFAPESP: 2018/15083-2-
Descrição: dc.descriptionFAPESP: 2021/13096-2-
Descrição: dc.descriptionCNPq: 23430/2021-1-
Descrição: dc.descriptionCNPq: 310049/2022-9-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: UID/04279-
Idioma: dc.languageen-
Relação: dc.relationScientifica-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectalkaline phosphatase-
Palavras-chave: dc.subjectcytotoxicity-
Palavras-chave: dc.subjectdentin mineralization-
Palavras-chave: dc.subjectflavonoids-
Palavras-chave: dc.subjectgene expression-
Título: dc.titleEGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast-Like Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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