Lymphocytic hypophysitis in dogs infected with Leishmania spp.

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorLyssavirus Epidemiology and Neuropathology Unit-
Autor(es): dc.contributorNorth Carolina State University-
Autor(es): dc.creatorFrigerio, Edenilson Doná-
Autor(es): dc.creatorGuizelini, Cecilia de Castro-
Autor(es): dc.creatorJussiani, Giulia Gonçalves-
Autor(es): dc.creatorMarço, Karen Santos-
Autor(es): dc.creatorde Melo, Guilherme Dias-
Autor(es): dc.creatorWatanabe, Tatiane Terumi Negrão-
Autor(es): dc.creatorMachado, Gisele Fabrino-
Data de aceite: dc.date.accessioned2025-08-21T17:05:21Z-
Data de disponibilização: dc.date.available2025-08-21T17:05:21Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fvets.2023.1208919-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299630-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299630-
Descrição: dc.descriptionBackground: Morphological involvement of endocrine glands, such as the pituitary gland, remain uninvestigated in dogs with canine visceral leishmaniasis. Therefore, this study investigated the presence of amastigotes of Leishmania spp. and characterized inflammatory changes, highlighting the involvement of TCD3+ lymphocytes in different regions of the pituitary gland of dogs. Methods: Samples were collected from 21 naturally infected dogs and 5 control, uninfected dogs. The different pituitary regions were analyzed in histological sections stained with hematoxylin and eosin (HE) under light microscopy. Inflammation was classified by intensity in a score from 0 to 3, absent (0), mild (1), moderate (2), and marked (3). The immunohistochemical (IHC) evaluation was performed in five high-power fields (hot spot) in a 40x objective of each region with manual counting (Image J1.52ª) of the TCD3+ lymphocytes and for amastigotes analyzed in 40x and 100x objectives. The Shapiro–Wilk test was used to assess the normality of the data. Differences between groups were determined by the Mann Whitney test. The correlation between variables was assessed by Sperman’s correlation test. p < 0.05 were considered statistically significant. Results: Amastigotes from the pituitary glands of two infected dogs were identified using IHC. The histopathological evaluation stained with hematoxylin and eosin showed greater intensity of inflammation in the pars distalis and pars intermedia regions of infected dogs. IHC for TCD3+ lymphocytes showed a higher median number of immunolabeled cells in pars nervosa in the infected group than in the control group (p < 0.05); and expecting a variation in the distribution and number of these cells in naturally infected dogs, the median of the control group was considered a cut-off point, an increase in T lymphocytes (p < 0.05) was also observed in the pars intermedia and pars distalis of an infected subgroup (n = 10). A moderate significant correlation between the intensity of inflammation and the number of immunolabeled TCD3+ lymphocytes was established in the analyzed pituitary regions, characterizing the occurrence of hypophysitis. Conclusion: These findings presuppose that inflammation and/or the parasite in the pituitary region can result in gland dysfunction, worsening the clinical condition of the patient and compromising the efficiency of treatment and prognosis.-
Descrição: dc.descriptionDepartment of Veterinary Clinics Surgery and Reproduction Faculty of Veterinary Medicine São Paulo State University UNESP-
Descrição: dc.descriptionInstitut Pasteur Université Paris Cité Lyssavirus Epidemiology and Neuropathology Unit-
Descrição: dc.descriptionDepartment Population Health and Pathobiology College of Veterinary Medicine North Carolina State University-
Descrição: dc.descriptionDepartment of Veterinary Clinics Surgery and Reproduction Faculty of Veterinary Medicine São Paulo State University UNESP-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Veterinary Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectimmunohistochemical-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectLeishmania infantum-
Palavras-chave: dc.subjectpituitary gland-
Palavras-chave: dc.subjectT lymphocyte-
Título: dc.titleLymphocytic hypophysitis in dogs infected with Leishmania spp.-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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