Use of coenzyme Q-10 to improve the pregnancy rate in sheep

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal University of Mato Grosso do Sul-
Autor(es): dc.creatorTironi, Stella Maris Teobaldo-
Autor(es): dc.creatorSitó-Silva, Luan-
Autor(es): dc.creatorde Camillo, Beatriz Lippe-
Autor(es): dc.creatorDenadai, Renan-
Autor(es): dc.creatorSilva, Adrielly Lais Alves da-
Autor(es): dc.creatorde Paula Freitas-Dell'Aqua, Camila-
Autor(es): dc.creatorJunior, José Antonio Dell'Aqua-
Autor(es): dc.creatorde Oliveira, Rogério Antonio-
Autor(es): dc.creatorSouza, Maria Inês Lenz-
Autor(es): dc.creatorOba, Eunice-
Data de aceite: dc.date.accessioned2025-08-21T18:37:43Z-
Data de disponibilização: dc.date.available2025-08-21T18:37:43Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2024.107498-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299572-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299572-
Descrição: dc.descriptionOne of the factors responsible for less pregnancy rates is the use of frozen semen in sheep due to the oxidative stress created by the process. The aim of this experiment was to test the effects of adding coenzyme Q-10 (CoQ10) to the seminal extender on sperm quality and the pregnancy rate of sheep. In this study, ejaculates from eight Dorper rams of reproductive age were used and tested in four treatments: Control (pure BotuBov®), C1 (175 µM of CoQ10), C3 (350 µM of CoQ10), and C7 (700 µM of CoQ10). Samples were collected in triplicate from each animal, and sperm analysis was performed by CASA after thawing at 0 h and 2 h. The samples were also analyzed by flow cytometry for plasma and acrosomal membrane integrity, stability, lipid peroxidation, mitochondrial potential, and superoxide anion production. In total, 198 ewes were inseminated by laparoscopy and divided into two groups: control (n=98) and C7 (n=100). Pregnancy diagnosis was performed at 30 days. Coenzyme Q10 proved to be safe for semen cryopreservation, not altering sperm kinetic values between the groups post-thawing. In flow cytometry, the C1 and C7 groups achieved a better index of plasma membrane integrity and membrane stability (P<0.05). A increased pregnancy rate was observed in C7 (52 %) compared to the control (38 %). In conclusion, coenzyme Q10 assists in the cryopreservation process, protecting the sperm cell and improving pregnancy rates in ewes.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionVeterinary Surgery and Animal Reproduction Department College of Veterinary Medicine and Animal Science Paulista State University-
Descrição: dc.descriptionAnimal Science Department Esalq-USP State Universitu of São Paulo-
Descrição: dc.descriptionBiostatistics Department Biosciences Institute Paulista State University-
Descrição: dc.descriptionMorphophysiology Department Biosciences Institute Federal University of Mato Grosso do Sul-
Descrição: dc.descriptionVeterinary Surgery and Animal Reproduction Department College of Veterinary Medicine and Animal Science Paulista State University-
Descrição: dc.descriptionBiostatistics Department Biosciences Institute Paulista State University-
Descrição: dc.descriptionCAPES: 001-
Idioma: dc.languageen-
Relação: dc.relationAnimal Reproduction Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntioxidant-
Palavras-chave: dc.subjectArtificial insemination-
Palavras-chave: dc.subjectCoQ10-
Palavras-chave: dc.subjectSemen cryopreservation-
Título: dc.titleUse of coenzyme Q-10 to improve the pregnancy rate in sheep-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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