PLGA/TPGS nanoparticles for docetaxel delivery: The pegylation effect on nanoparticle physicochemical properties and uptake and cytotoxicity in prostate cancer cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorDentistry and Nursing-
Autor(es): dc.creatorSilva, Lívia de Queiróz Carvalho-
Autor(es): dc.creatorRaspantini, Giovanni Loureiro-
Autor(es): dc.creatorAbriata, Juliana Palma-
Autor(es): dc.creatorLuiz, Marcela Tavares-
Autor(es): dc.creatorde Sousa, Ana Carolina Cruz-
Autor(es): dc.creatorMoreira, Thais da Silva-
Autor(es): dc.creatorMagalhães, Emanuel Paula-
Autor(es): dc.creatorde Menezes, Ramon Róseo Paula Pessoa Bezerra-
Autor(es): dc.creatorPetrilli, Raquel-
Autor(es): dc.creatorMarchetti, Juliana Maldonado-
Autor(es): dc.creatorEloy, Josimar O.-
Data de aceite: dc.date.accessioned2025-08-21T17:54:43Z-
Data de disponibilização: dc.date.available2025-08-21T17:54:43Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.xphs.2025.103766-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299532-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299532-
Descrição: dc.descriptionProstate cancer is the most common malignancy in men worldwide and docetaxel (DTX) is the treatment of choice. However, both the drug and formulation excipients for drug solubilization can cause side effects. In this context, the development of polymeric nanoparticles offers advantages to improve drug delivery and reduce toxicity. In the present work, factorial design was used to evaluate the effect of the amount of poly(L-lactide-co-glycolide) (PLGA) or poly(L-lactide-co-glycolide acid-polyethylene glycol) (PLGA-PEG), D-Alpha-Tocopheryl Polyethylene Glycol Succinate (TPGS) and ratio between aqueous and oily phases on the nanoparticle characteristics. The nanocarriers were characterized regarding particle size, polydispersity, zeta potential, DTX encapsulation efficiency, morphology by transmission electron microscopy, DSC, TGA and FTIR. It was evaluated in vitro for cytotoxicity and cellular uptake in prostate cancer cells. Pegylated nanoparticles, which have a different composition (TPGS%, AP:OP ratio), reduced the nanoparticle size to 105.97 ± 5.16 nm, in PDI 0.13 ± 0.03, zeta potential of -34.73 ± 1.19 mV and increased the encapsulation efficiency to 96.78 ± 1.20%. Characterization by DSC, TGA and FTIR confirmed drug encapsulation and showed colloidal stability. Pegylated nanoparticles were more stable upon serum incubation and adsorbed less proteins. In conclusion, the pegylation of the nanoparticles affected the physicochemical parameters. Also, the pegylation of nanoparticles decreased uptake by macrophages. Finally, cellular uptake and cell cytotoxicity were higher in tumor cells when compared to non-tumor cells, although they were not affected by pegylation.-
Descrição: dc.descriptionUniversity of Sao Paulo Faculty of Pharmaceutical Sciences of Ribeirão Preto, SP-
Descrição: dc.descriptionPaulista State University Faculty of Pharmaceutical Sciences, SP-
Descrição: dc.descriptionFederal University of Ceará Faculty of Pharmacy Dentistry and Nursing Department of Pharmacy, CE-
Descrição: dc.descriptionPaulista State University Faculty of Pharmaceutical Sciences, SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Pharmaceutical Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDocetaxel-
Palavras-chave: dc.subjectPegylation-
Palavras-chave: dc.subjectPLGA-
Palavras-chave: dc.subjectProstate cancer-
Palavras-chave: dc.subjectTPGS-
Título: dc.titlePLGA/TPGS nanoparticles for docetaxel delivery: The pegylation effect on nanoparticle physicochemical properties and uptake and cytotoxicity in prostate cancer cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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