MPO interacts with hRSV particles, contributing to the virucidal effects of NETs against clinical and laboratory hRSV isolates

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Jatai-
Autor(es): dc.contributorUniversidade Nova de Lisboa-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorEscola de Engenharia da Universidade do Minho-
Autor(es): dc.creatorda Silva Pinto, Leonardo-
Autor(es): dc.creatorJunior, Ronaldo Silva Alves-
Autor(es): dc.creatorLopes, Bruno Rafael Pereira-
Autor(es): dc.creatorda Silva, Gabriel Soares-
Autor(es): dc.creatorde Lima Menezes, Gabriela-
Autor(es): dc.creatorMoreira, Pedro-
Autor(es): dc.creatorde Oliveira, Juliana-
Autor(es): dc.creatorda Silva, Roosevelt Alves-
Autor(es): dc.creatorLousa, Diana-
Autor(es): dc.creatorToledo, Karina Alves-
Data de aceite: dc.date.accessioned2025-08-21T20:28:27Z-
Data de disponibilização: dc.date.available2025-08-21T20:28:27Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2024.137423-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299511-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299511-
Descrição: dc.descriptionHuman Respiratory syncytial virus (hRSV) mainly affects immunosuppressed patients requiring hospitalization. No specific treatment is financially accessible, and available vaccines do not cover all risk groups. During hRSV infection, there is a robust neutrophilic influx into the airways. hRSV-activated neutrophils release substantial neutrophil extracellular traps (NETs) in lung tissue, comprising DNA, histones, cytosolic, and granular proteins. NETs form mucus buildup in the lungs, compromising respiratory capacity and neutralizing viral particles. Understanding responsible NETs molecules requires improvement. We evaluated NETs interacting with hRSV particles and their contribution to anti-hRSV NET effects. Immunoblotting, immunoprecipitation, and peptide sequencing assays confirmed hRSV binding to a 50–75 kDa NET protein, Myeloperoxidase (MPO). MPO, a microbicide enzyme in NETs, interacts with hRSV, likely at F0 protein (site IV) on the viral surface. Additionally, MPO (32 μM) and NETs (0.4 μg/mL) reduced in vitro replication of clinical (hRSV A and B) and laboratory (Long) hRSV isolates by approximately 30 %, reversible by selective MPO inhibitor (PF-06281355; 48 μM). Thus, MPO contributes to virucidal NET effects on diverse hRSV strains, enhancing comprehension of NETs' role in infection and aiding treatment strategies for respiratory diseases.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionEuropean Commission-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences, SP-
Descrição: dc.descriptionBiosystems Collaborative Nucleus Institute of Exact Sciences Federal University of Jatai-
Descrição: dc.descriptionInstituto de Tecnologia Química e Biológica António Xavier Universidade Nova de Lisboa-
Descrição: dc.descriptionGraduate Program in Applied and Computational Mathematics – PGMAC – State University of Londrina, PR-
Descrição: dc.descriptionCentro de Engenharia Biológica Escola de Engenharia da Universidade do Minho-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences, SP-
Descrição: dc.descriptionEuropean Commission: 101137419-
Descrição: dc.descriptionFAPESP: 2018/09021-4-
Descrição: dc.descriptionFAPESP: 2021/05107-4-
Descrição: dc.descriptionFAPESP: 2022/14220-1-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntiviral-
Palavras-chave: dc.subjectEnzyme-
Palavras-chave: dc.subjectMicrobicidal-
Palavras-chave: dc.subjectNeutrophils-
Palavras-chave: dc.subjectSyncytial-
Título: dc.titleMPO interacts with hRSV particles, contributing to the virucidal effects of NETs against clinical and laboratory hRSV isolates-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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