Time-efficient germ cell transplantation from goldfish (Carassius auratus) into adult common carp (Cyprinus carpio)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFundación Universitaria San Martín-
Autor(es): dc.contributorChinese Academy of Fishery Sciences-
Autor(es): dc.creatorVigoya, Angel Andreas Arias-
Autor(es): dc.creatorCosta, Daniel Fernandes da-
Autor(es): dc.creatorOliveira, Marcos Antônio de-
Autor(es): dc.creatorButzge, Arno Juliano-
Autor(es): dc.creatorRosa, Ivana Felipe-
Autor(es): dc.creatorDoretto, Lucas Benites-
Autor(es): dc.creatorMartinez, Emanuel Ricardo Monteiro-
Autor(es): dc.creatorDigmayer, Melanie-
Autor(es): dc.creatorNóbrega, Rafael Henrique-
Data de aceite: dc.date.accessioned2025-08-21T18:31:18Z-
Data de disponibilização: dc.date.available2025-08-21T18:31:18Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1984-3143-AR2023-0121-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299506-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299506-
Descrição: dc.descriptionGerm cell transplantation in fish is a promising technique for surrogate broodstock parents with broader application in aquaculture and conserving endangered and valuable genetic resources. Herein, we describe the establishment of an intrapapillary xenogeneic transplant of germ cells from sexually mature goldfish (C. auratus) males into common carp (C. carpio) males cytoablated with a thermochemical treatment (two doses of busulfan at 40 mg/kg at 35°C). To analyze the presence and development of donor germ cells in recipient testes, donor germ cells were labeled with PKH26, a fluorescent cell membrane dye, before transplantation. Our results demonstrated that thermochemical treatment caused effective spermatogenesis suppression and pronounced germ cell loss. Moreover, transplanted spermatogonial cells were able to colonize the recipients’ testes, resume spermatogenesis, and generate spermatozoa within eight weeks after germ cell transplantation. These findings suggested that recipient testes provided suitable conditions for the survival, colonization, proliferation, and differentiation of donor spermatogonia from a related species. This study indicated that recipients’ testes exhibited a high degree of plasticity to accept and support xenogeneic donor germ cells, which were able to form sperm in a short time frame. This approach has significant implications for assisted animal reproduction, biotechnology, conservation, and the production of valuable genetic resources and endangered fish species.-
Descrição: dc.descriptionCentro de Aquicultura Universidade Estadual Paulista, Jaboticabal-
Descrição: dc.descriptionFacultad de Medicina Veterinaria y Zootecnia Fundación Universitaria San Martín-
Descrição: dc.descriptionDepartamento de Biologia Estrutural e Funcional Instituto de Biosciências Universidade Estadual Paulista, SP-
Descrição: dc.descriptionNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences-
Descrição: dc.descriptionCentro de Aquicultura Universidade Estadual Paulista, Jaboticabal-
Descrição: dc.descriptionDepartamento de Biologia Estrutural e Funcional Instituto de Biosciências Universidade Estadual Paulista, SP-
Idioma: dc.languageen-
Relação: dc.relationAnimal Reproduction-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiotechnology-
Palavras-chave: dc.subjectcommon carp-
Palavras-chave: dc.subjectgoldfish-
Palavras-chave: dc.subjectspermatogenesis-
Palavras-chave: dc.subjectspermatogonial stem cells-
Palavras-chave: dc.subjectteleost fish-
Título: dc.titleTime-efficient germ cell transplantation from goldfish (Carassius auratus) into adult common carp (Cyprinus carpio)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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