Bifunctional naringenin-laden gelatin methacryloyl scaffolds with osteogenic and anti-inflammatory properties

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Michigan–School of Dentistry-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorStony Brook University–School of Dental Medicine-
Autor(es): dc.contributorUniversity of Michigan-
Autor(es): dc.creatorCardoso, Lais M.-
Autor(es): dc.creatorde Carvalho, Ana Beatriz G.-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorMahmoud, Abdel H.-
Autor(es): dc.creatorDal-Fabbro, Renan-
Autor(es): dc.creatorBasso, Fernanda G.-
Autor(es): dc.creatorBottino, Marco C.-
Data de aceite: dc.date.accessioned2025-08-21T16:00:22Z-
Data de disponibilização: dc.date.available2025-08-21T16:00:22Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dental.2024.06.019-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299493-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299493-
Descrição: dc.descriptionObjective: To fabricate and characterize an innovative gelatin methacryloyl/GelMA electrospun scaffold containing the citrus flavonoid naringenin/NA with osteogenic and anti-inflammatory properties. Methods: GelMA scaffolds (15 % w/v) containing 0/Control, 5, 10, or 20 % of NA w/w were obtained via electrospinning. The chemical composition, fiber morphology/diameter, swelling/degradation profile, and NA release were investigated. Cytotoxicity, cell proliferation, adhesion and spreading, total protein/TP production, alkaline phosphatase/ALP activity, osteogenic genes expression (OCN, OPN, RUNX2), and mineralized nodules deposition/MND with human alveolar bone-derived mesenchymal stem cells (aBMSCs) seeded on the scaffolds were assessed. Moreover, aBMSCs seeded on the scaffolds and stimulated with tumor necrosis factor-alpha/TNF-α were submitted to collagen, nitric oxide/NO, interleukin/IL-1α, and IL-6 production assessment. Data were analyzed using ANOVA and t-student/post-hoc tests (α = 5 %). Results: NA-laden scaffolds presented increased fiber diameter, lower swelling capacity, and faster degradation profile over 28 days (p < 0.05). NA release was detected over time. Cell adhesion and spreading, and TP production were similar between GelMA and GelMA+NA5 % scaffolds, while cell proliferation, ALP activity, OCN/OPN/RUNX2 gene expression, and MND were higher for GelMA+NA5 % scaffolds (p < 0.05). Cells seeded on control scaffolds and TNF-α-stimulated presented higher levels of NO, IL-1α/IL-6, and lower levels of collagen (p < 0.05). In contrast, cells seeded on GelMA+NA5 % scaffolds showed downregulation of inflammatory markers and higher collagen synthesis (p < 0.05). Significance: GelMA+NA5 % scaffold was cytocompatible, stimulated aBMSCs proliferation and differentiation, and downregulated inflammatory mediators’ synthesis, suggesting its therapeutic effect as a multi-target bifunctional scaffold with osteogenic and anti-inflammatory properties for bone tissue engineering.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionNational Institutes of Health-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Cariology Restorative Sciences and Endodontics University of Michigan–School of Dentistry, 1011 N. University Avenue-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP)–São Jose dos Campos School of Dentistry, Eng. Francisco Jose Longo 777, SP-
Descrição: dc.descriptionDepartment of Morphology and Pediatric Dentistry São Paulo State University (UNESP)–Araraquara School of Dentistry, Humaitá 1680, SP-
Descrição: dc.descriptionDepartment of Oral Biology and Pathology Stony Brook University–School of Dental Medicine, 100 Nicolls Road-
Descrição: dc.descriptionDepartment of Phisiology and Pathology São Paulo State University (UNESP) Araraquara School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Biomedical Engineering College of Engineering University of Michigan-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP)–São Jose dos Campos School of Dentistry, Eng. Francisco Jose Longo 777, SP-
Descrição: dc.descriptionDepartment of Morphology and Pediatric Dentistry São Paulo State University (UNESP)–Araraquara School of Dentistry, Humaitá 1680, SP-
Descrição: dc.descriptionDepartment of Phisiology and Pathology São Paulo State University (UNESP) Araraquara School of Dentistry, SP-
Descrição: dc.descriptionFAPESP: #2019/16886-4-
Descrição: dc.descriptionFAPESP: #2022/03788-7-
Descrição: dc.descriptionCNPq: 303574/2022-4-
Formato: dc.format1353-1363-
Idioma: dc.languageen-
Relação: dc.relationDental Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBone-
Palavras-chave: dc.subjectElectrospinning-
Palavras-chave: dc.subjectFlavonoids-
Palavras-chave: dc.subjectGelatin methacryloyl-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectRegeneration-
Título: dc.titleBifunctional naringenin-laden gelatin methacryloyl scaffolds with osteogenic and anti-inflammatory properties-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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