Fish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology

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Autor(es): dc.contributorFederal University of Rio Grande do Sul-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCatholic University of Temuco-
Autor(es): dc.creatorde Souza França, Thales-
Autor(es): dc.creatorGomes, Itamar Cossina-
Autor(es): dc.creatorSanches, Eduardo Antônio-
Autor(es): dc.creatorAtehortúa, Maritza Pérez-
Autor(es): dc.creatordos Santos Teixeira, Nathalia-
Autor(es): dc.creatorRodrigues, Rômulo Batista-
Autor(es): dc.creatorde Freitas, Thaiza Rodrigues-
Autor(es): dc.creatorBenato, Jhony Lisbôa-
Autor(es): dc.creatorMarques, Lis Santos-
Autor(es): dc.creatorde Souza, Ana Regina Seabra-
Autor(es): dc.creatorStreit, Danilo Pedro-
Data de aceite: dc.date.accessioned2025-08-21T21:56:44Z-
Data de disponibilização: dc.date.available2025-08-21T21:56:44Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1530/REP-23-0046-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299480-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299480-
Descrição: dc.descriptionContainers used to cryopreserve sperm are made with non-biodegradable plastic compounds, having a high monetary and environmental cost. Therefore, the development of biodegradable alternative containers for cell cryopreservation is necessary. Thus, this study aimed to evaluate the efficiency of hard-gelatin and hard-hydroxypropyl methylcellulose (HPMC) capsules as low-cost and biodegradable alternative containers for sperm cryopreservation. Sperm from 12South American silver catfish Rhamdia quelen were individually cryopreserved in plastic straws 0.25 mL (as control), hard-gelatin, and hard-HPMC capsules. The quality of post-thaw sperm cryopreserved in the different containers was checked by measuring spermatozoa membrane integrity, kinetic parameters, mitochondrial activity, fertilization, hatching, and normal larvae rates. The samples cryopreserved in straws showed a higher percentage of membrane integrity (68%) than those frozen in hard-gelatin (40%) and hard-HPMC capsules (40%). However, we did not observe differences between the samples stored in straws and hard capsules for the rest of the tested sperm parameters. Thus, based on the high sperm fertility capability, both capsules were efficient as cryopreservation containers for maintaining sperm functionality.-
Descrição: dc.descriptionAquam Research Group Animal Science Research Program Federal University of Rio Grande do Sul, RS-
Descrição: dc.descriptionFishery Engineering Course and Aquaculture Centre (CAUNESP) São Paulo State University, Registro-
Descrição: dc.descriptionDepartment of Agricultural and Aquacultural Sciences Faculty of Natural Resources Catholic University of Temuco-
Descrição: dc.descriptionPostgraduate Program in Veterinary Science Federal University of Rio Grande do Sul, RS-
Descrição: dc.descriptionFishery Engineering Course and Aquaculture Centre (CAUNESP) São Paulo State University, Registro-
Formato: dc.format89-97-
Idioma: dc.languageen-
Relação: dc.relationReproduction-
???dc.source???: dc.sourceScopus-
Título: dc.titleFish sperm cryopreservation using biodegradable containers: New low-cost and environment-friendly methodology-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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