Plasma eNOS Concentration in Healthy Pregnancy and in Hypertensive Disorders of Pregnancy: Evidence of Reduced Concentrations in Pre-Eclampsia from Two Independent Studies

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Mississippi Medical Center-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.creatorKaihara, Julyane N. S.-
Autor(es): dc.creatorMinami, Caroline K.-
Autor(es): dc.creatorPeraçoli, Maria T. S.-
Autor(es): dc.creatorRomão-Veiga, Mariana-
Autor(es): dc.creatorRibeiro-Vasques, Vanessa R.-
Autor(es): dc.creatorPeraçoli, José C.-
Autor(es): dc.creatorPalei, Ana C. T.-
Autor(es): dc.creatorCavalli, Ricardo C.-
Autor(es): dc.creatorNunes, Priscila R.-
Autor(es): dc.creatorLuizon, Marcelo R.-
Autor(es): dc.creatorSandrim, Valeria C.-
Data de aceite: dc.date.accessioned2025-08-21T16:08:33Z-
Data de disponibilização: dc.date.available2025-08-21T16:08:33Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/diseases11040155-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299451-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299451-
Descrição: dc.descriptionHypertensive disorders of pregnancy (HDP), comprising gestational hypertension (GH) and pre-eclampsia (PE), are leading causes of maternal and perinatal morbidity and mortality. Both GH and PE are characterized by new-onset hypertension, but PE additionally includes proteinuria and/or end-organ damage. Impaired nitric oxide (NO) bioavailability may lead to endothelial dysfunction in GH and PE, and the primary source of vascular NO is endothelial NO synthase (eNOS). However, no previous study has investigated plasma eNOS concentrations in patients with GH and PE. In this study, we compared plasma eNOS concentrations in healthy pregnancies and HDP in two independent cohorts. The primary study included 417 subjects, with 43 non-pregnant (NP) and 156 healthy pregnant (HP) women and 122 patients with GH and 96 with PE. The replication study included 85 pregnant women (41 healthy and 44 pre-eclamptic). Plasma concentrations of eNOS were measured using a commercial ELISA kit provided by R&D Systems, and plasma nitrite concentrations were assessed using two ozone-based chemiluminescence assays. Correlations between plasma eNOS concentrations and plasma nitrite concentrations, as well as clinical and biochemical parameters, were evaluated by either Spearman’s or Pearson’s tests. In the primary study, NP women and HDP had significantly lower plasma eNOS concentrations compared to HP; concentrations were even lower in PE compared to GH. Plasma eNOS concentrations were reduced but not significant in early-onset PE, PE with severe features, preterm birth, and intrauterine growth restriction. No correlation was observed between plasma eNOS and nitrite levels. In HDP, there was a significant positive correlation between levels of eNOS and hemoglobin (r = 0.1496, p = 0.0336) as well as newborn weight (r = 0.1487, p = 0.0316). Conversely, a negative correlation between eNOS levels and proteinuria was observed (r = −0.2167, p = 0.0179). The replication study confirmed significantly reduced plasma concentrations of eNOS in PE compared to HP. Our findings provide evidence of reduced plasma eNOS concentrations in HDP; they were particularly lower in PE compared to GH and HP in two independent studies.-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemistry and Biological Sciences Institute of Biosciences of Botucatu Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Surgery School of Medicine University of Mississippi Medical Center-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Faculty of Medicine of Ribeirao Preto University of Sao Paulo (USP), SP-
Descrição: dc.descriptionDepartment of Genetics Ecology and Evolution Institute of Biological Sciences Federal University of Minas Gerais, MG-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Chemistry and Biological Sciences Institute of Biosciences of Botucatu Sao Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationDiseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectendothelial nitric oxide synthase-
Palavras-chave: dc.subjectgestational hypertension-
Palavras-chave: dc.subjectnitric oxide-
Palavras-chave: dc.subjectNOS3 protein-
Palavras-chave: dc.subjectpre-eclampsia-
Palavras-chave: dc.subjectpregnancy-
Título: dc.titlePlasma eNOS Concentration in Healthy Pregnancy and in Hypertensive Disorders of Pregnancy: Evidence of Reduced Concentrations in Pre-Eclampsia from Two Independent Studies-
Tipo de arquivo: dc.typelivro digital-
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