Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Saskatchewan-
Autor(es): dc.creatorDias, Luana Mendonça-
Autor(es): dc.creatorCilli, Eduardo Maffud-
Autor(es): dc.creatorMedeiros, Karine Sousa-
Autor(es): dc.creatorBrasil, Maria Carolina Oliveira de Arruda-
Autor(es): dc.creatorMarin, Lina Maria-
Autor(es): dc.creatorSiqueira, Walter L.-
Autor(es): dc.creatorPavarina, Ana Claudia-
Data de aceite: dc.date.accessioned2025-08-21T19:21:29Z-
Data de disponibilização: dc.date.available2025-08-21T19:21:29Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/microorganisms12010099-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299396-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299396-
Descrição: dc.descriptionThe aim of the study was to investigate the effect of antimicrobial peptides (AMPs) Hylin−a1, KR−12-a5, and Temporin-SHa in Candida albicans as well as the biocompatibility of keratinocytes spontaneously immortalized (NOK-si) and human gingival fibroblasts (FGH) cells. Initially, the susceptible (CaS—ATCC 90028) and fluconazole-resistant (CaR—ATCC 96901) C. albicans strains were grown to evaluate the effect of each AMP in planktonic culture, biofilm, and biocompatibility on oral cells. Among the AMPs evaluated, temporin−SHa showed the most promising results. After 24 h of Temporin-SHa exposure, the survival curve results showed that CaS and CaR suspensions reduced 72% and 70% of cell viability compared to the control group. The minimum inhibitory/fungicide concentrations (MIC and MFC) showed that Temporin−SHa was able to reduce ≥50% at ≥256 µg/mL for both strains. The inhibition of biofilm formation, efficacy against biofilm formation, and total biomass assays were performed until 48 h of biofilm maturation, and Temporin-SHa was able to reduce ≥50% of CaS and CaR growth. Furthermore, Temporin−SHa (512 µg/mL) was classified as non-cytotoxic and slightly cytotoxic for NOK-si and FGH, respectively. Temporin−SHa demonstrated an anti-biofilm effect against CaS and CaR and was biocompatible with NOK-si and FGH oral cells in monolayer.-
Descrição: dc.descriptionCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada-
Descrição: dc.descriptionCanadian Institutes of Health Research-
Descrição: dc.descriptionCanadian HIV Trials Network, Canadian Institutes of Health Research-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionCollege of Dentistry University of Saskatchewan-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada: 2020/06119-
Descrição: dc.descriptionCanadian Institutes of Health Research: MOP-106657-
Descrição: dc.descriptionCanadian HIV Trials Network, Canadian Institutes of Health Research: MOP-106657-
Descrição: dc.descriptionCanadian Institutes of Health Research: PJT-159760-
Descrição: dc.descriptionCanadian HIV Trials Network, Canadian Institutes of Health Research: PJT-159760-
Idioma: dc.languageen-
Relação: dc.relationMicroorganisms-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantimicrobial peptides-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectfibroblasts-
Palavras-chave: dc.subjectkeratinocytes-
Título: dc.titleAntibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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