Isoflurane increases the activity of the vascular matrix metalloproteinase-2 in non-pregnant rats and increases the nitric oxide metabolites in pregnancy

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUNAERP-
Autor(es): dc.creatorSouza, Carolina Rosa Rodrigues-
Autor(es): dc.creatorCaetano, Edileia Souza Paula-
Autor(es): dc.creatorRodrigues, Serginara David-
Autor(es): dc.creatorLopes, Matheus Cleto-
Autor(es): dc.creatorMattos, Bruna Rahal-
Autor(es): dc.creatorSantos, Mariana Landenberger-
Autor(es): dc.creatorRizzi, Elen-
Autor(es): dc.creatorDias, Carlos A.-
Data de aceite: dc.date.accessioned2025-08-21T21:56:52Z-
Data de disponibilização: dc.date.available2025-08-21T21:56:52Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1042/BSR20240192-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/299386-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/299386-
Descrição: dc.descriptionSurgeries that require general anesthesia occur in 1.5–2% of gestations. Isoflurane is frequently used because of its lower possibility of affecting fetal growth. Therefore, we examined the isoflurane anesthesia-induced effects on maternal hemodynamic and vascular changes. We hypothesized that isoflurane would enhance endothelium-dependent vasodilation as a consequence of increased nitric oxide and decreased metalloproteinases (MMPs). Female rats (n=28) were randomized into 4 groups (7 rats/group): conscious (non-anesthetized) non-pregnant group, non-pregnant anesthetized group, conscious pregnant group, and pregnant anesthetized group. Anesthesia was performed on the 20th pregnancy day, and hemodynamic parameters were monitored. Nitric oxide metabolites, gelatinolytic activity of MMP-2 and MMP-9, and the vascular function were assessed. Isoflurane caused no significant hemodynamic changes in pregnant compared with non-pregnant anesthetized group. Impaired acetylcholine-induced relaxations were observed only in conscious non-pregnant group (by approximately 62%) versus 81% for other groups. Phenylephrine-induced contractions were greater in endothelium-removed aorta segments of both pregnant groups (with or without isoflurane) compared with non-pregnant groups. Higher nitric oxide metabolites were observed in anesthetized pregnant in comparison with the other groups. Reductions in the 75 kDa activity and concomitant increases in 64 kDa MMP-2 isoforms were observed in aortas of pregnant anesthetized (or not) groups compared with conscious non-pregnant group. Isoflurane anesthesia shows stable effects on hemodynamic parameters and normal MMP-2 activation in pregnancy. Furthermore, there were increases in nitric oxide bioavailability, suggesting that isoflurane provides protective actions to the endothelium in pregnancy.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science UNESP, Sao Paulo-
Descrição: dc.descriptionUnit of Biotechnology University of Ribeirao Preto UNAERP, Ribeirao Preto-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Sao Paulo State University (UNESP), Sao Paulo-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science UNESP, Sao Paulo-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2018/24.484-0-
Descrição: dc.descriptionFAPESP: 2019/06.072-0-
Descrição: dc.descriptionFAPESP: 2021/08.380-3-
Descrição: dc.descriptionCNPq: 406442/2022-3-
Descrição: dc.descriptionCNPq: CNPq—305091/2021-
Idioma: dc.languageen-
Relação: dc.relationBioscience Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleIsoflurane increases the activity of the vascular matrix metalloproteinase-2 in non-pregnant rats and increases the nitric oxide metabolites in pregnancy-
Tipo de arquivo: dc.typelivro digital-
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